Brusick D, Myhr B, Galloway S, Rundell J, Jagannath D R, Tarka S
Mutat Res. 1986 Mar;169(3):115-21. doi: 10.1016/0165-1218(86)90090-x.
Cocoa powder was evaluated for genotoxic activity and found to be inactive in the Ames assay, the mouse lymphoma assay, cytogenetic assays measuring chromosome breakage and SCE, and a cell transformation assay using Balb/c-3T3 cells. Although pure theobromine has been shown to be active in some of these test procedures, the levels of this methylxanthine present in cocoa powder were insufficient to elicit responses in this battery of tests.
对可可粉的遗传毒性活性进行了评估，结果发现在艾姆斯试验、小鼠淋巴瘤试验、检测染色体断裂和姐妹染色单体交换的细胞遗传学试验以及使用Balb/c - 3T3细胞的细胞转化试验中，可可粉均无活性。尽管已证明纯可可碱在其中一些测试程序中具有活性，但可可粉中这种甲基黄嘌呤的含量不足以在这一系列测试中引发反应。
