Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163000, Heilongjiang Province, China; School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, Jiangsu Province, China.
Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163000, Heilongjiang Province, China.
Parasitol Int. 2022 Jun;88:102555. doi: 10.1016/j.parint.2022.102555. Epub 2022 Feb 5.
Fascioliasis is a foodborne zoonotic disease generally caused by the parasitic flukes Fasciola gigantica and Fasciola hepatica in class Trematoda. An "intermediate" Fasciola forms between F. gigantica and F. hepatica has been shown to exist. However, the relationships among F. gigantica, F. hepatica, and "intermediate" Fasciola forms remain unclear. In this study, we found five new polymorphic positions in 18S and 28S rDNAs sequences of "intermediate" Fasciola forms. According to the high-throughput sequencing results, all known 16 polymorphic positions of "intermediate" Fasciola forms show a clear and consistent tendency for F. gigantica or F. hepatica, and the percentages of the most frequently occurring bases were different in specimens. In the three ITS sequence fragments, hybrid-type base combinations of the polymorphic positions were detected, and the percentages of the most frequent base combinations were different in specimens too. In addition, interestingly, the newly detected ITS-802 position was not a traditional polymorphic position in "intermediate" Fasciola forms, and the bases in ITS-802 position are not same as the allele bases of F. gigantica or F. hepatica. Our results will be helpful to investigations into the molecular taxonomy, population genetics, and ecology of F. gigantica, F. hepatica, and "intermediate" Fasciola forms.
片形吸虫病是一种食源性病原体引起的人畜共患疾病，通常由吸虫纲的巨型片形吸虫和肝片形吸虫引起。已经证明存在一种“中间”的片形吸虫。然而，巨型片形吸虫、肝片形吸虫和“中间”片形吸虫之间的关系仍不清楚。在这项研究中，我们在“中间”片形吸虫的 18S 和 28S rDNA 序列中发现了五个新的多态性位置。根据高通量测序结果，“中间”片形吸虫所有已知的 16 个多态性位置都表现出明显一致的巨型片形吸虫或肝片形吸虫倾向，且标本中最常出现的碱基百分比不同。在三个 ITS 序列片段中，检测到多态性位置的杂交型碱基组合，且标本中最常见碱基组合的百分比也不同。此外，有趣的是，新检测到的 ITS-802 位置不是“中间”片形吸虫中的传统多态性位置，并且 ITS-802 位置的碱基与巨型片形吸虫或肝片形吸虫的等位基因碱基不同。我们的研究结果将有助于对巨型片形吸虫、肝片形吸虫和“中间”片形吸虫的分子分类学、群体遗传学和生态学的研究。
