Toulmé J J, Krisch H M, Loreau N, Thuong N T, Hélène C
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1227-31. doi: 10.1073/pnas.83.5.1227.
Synthetic oligodeoxynucleotides that are covalently linked at their 3' end to an acridine derivative and are complementary to the repeated sequence UUAAAUUAAAUUAAA adjacent to the ribosome binding site of the gene 32-encoded mRNA from phage T4 have been used to regulate the synthesis of gene 32-encoded protein in vitro. These modified, synthetic oligonucleotides specifically block the translation of gene 32-encoded mRNA with a higher efficiency than the homologous unsubstituted oligonucleotides. The inhibition produced by these short "anti-messengers" is due to the formation of specific mRNA . oligodeoxynucleotide hybrids that are stabilized by the intercalation of the acridine ring in the RNA . DNA duplex.
在其3'端与吖啶衍生物共价连接且与噬菌体T4基因32编码的mRNA的核糖体结合位点相邻的重复序列UUAAAUUAAAUUAAA互补的合成寡脱氧核苷酸已被用于体外调节基因32编码蛋白的合成。这些修饰的合成寡核苷酸比同源未取代的寡核苷酸更有效地特异性阻断基因32编码的mRNA的翻译。这些短的“反信使”产生的抑制作用是由于形成了特定的mRNA-寡脱氧核苷酸杂交体，该杂交体通过吖啶环插入RNA-DNA双链体而得以稳定。
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