Asseline U, Nguyen T T, Hélène C
C R Seances Acad Sci III. 1983;297(7):369-72.
New molecules are described in which an intercalating agent is covalently linked through a polymethylene chain to an oligodeoxynucleotide. Using an oligodT sequence covalently linked to an acridine dye, it is shown that these molecules bind in a specific way to their complementary sequence (poly (A] and that the stability is strongly enhanced by the intercalating agent as compared with the unmodified oligodT. Substitution of a phosphate group by a positively charged group strengthens complex formation and protects the neighboring phosphodiester bond against nuclease cleavage.
本文描述了一类新分子，其中嵌入剂通过聚亚甲基链与寡脱氧核苷酸共价连接。使用与吖啶染料共价连接的寡聚dT序列，结果表明这些分子以特定方式与其互补序列（聚A）结合，并且与未修饰的寡聚dT相比，嵌入剂可显著增强其稳定性。用带正电荷的基团取代磷酸基团可增强复合物的形成，并保护相邻的磷酸二酯键不被核酸酶切割。
