Department of Clinical Medicine, Guizhou Medical University, Guiyang, China.
The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Biomed Res Int. 2022 Jan 28;2022:9868022. doi: 10.1155/2022/9868022. eCollection 2022.
Hepatocellular carcinoma (HCC) is widely acknowledged as a malignant tumor with rapid progression, high recurrence rate, and poor prognosis. At present, there is a paucity of reliable biomarkers at the clinical level to guide the management of HCC and improve patient outcomes. Our research is aimed at assessing the prognostic value of MAD2L1 in HCC.
Four datasets, GSE121248, GSE101685, GSE85598, and GSE62232, were selected from the GEO database to analyze differentially expressed genes (DEGs) between HCC and normal liver tissues. After functional analysis, we constructed a protein-protein interaction network (PPI) for DEGs and identified core genes in this network with high connectivity with other genes. We assessed the relationship between core genes and the pathogenesis and prognosis of HCC. Finally, we explored the gene regulatory signaling mechanisms involved in HCC pathogenesis.
145 DEGs were screened from the intersection of the four GEO datasets. MAD2L1 was associated with most genes according to the PPI network and was selected as a candidate gene for further study. Survival analysis suggested that high MAD2L1 expression in HCC correlated with a worse prognosis. In addition, real-time quantitative PCR (RT-qPCR), western blot (WB), and immunohistochemistry (IHC) findings suggested that the expression of MAD2L1 was abnormally increased in HCC tissues and cells compared to paraneoplastic tissues and normal hepatocytes.
We found that high MAD2L1 expression in HCC was significantly associated with overall patient survival and clinical features. We also explored the potential biological properties of this gene.
肝细胞癌（HCC）是一种恶性肿瘤，其进展迅速、复发率高、预后差。目前，临床上缺乏可靠的生物标志物来指导 HCC 的管理和改善患者的预后。我们的研究旨在评估 MAD2L1 在 HCC 中的预后价值。
从 GEO 数据库中选择了四个数据集 GSE121248、GSE101685、GSE85598 和 GSE62232，以分析 HCC 和正常肝组织之间的差异表达基因（DEGs）。在功能分析之后，我们构建了 DEGs 的蛋白质-蛋白质相互作用网络（PPI），并确定了该网络中与其他基因具有高连接性的核心基因。我们评估了核心基因与 HCC 发病机制和预后的关系。最后，我们探讨了参与 HCC 发病机制的基因调控信号机制。
从四个 GEO 数据集的交集筛选出 145 个 DEGs。根据 PPI 网络，MAD2L1 与大多数基因相关，被选为进一步研究的候选基因。生存分析表明，HCC 中 MAD2L1 的高表达与预后较差相关。此外，实时定量 PCR（RT-qPCR）、蛋白质印迹（WB）和免疫组织化学（IHC）的结果表明，与旁瘤组织和正常肝细胞相比，MAD2L1 在 HCC 组织和细胞中的表达异常升高。
我们发现 HCC 中 MAD2L1 的高表达与患者的总体生存和临床特征显著相关。我们还探讨了该基因的潜在生物学特性。
