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[电离辐射对大鼠肝脏赖氨酰tRNA合成酶甲基化的影响]

[Effect of ionizing radiation on methylation of lysyl tRNA synthetase from rat liver].

作者信息

Vinogradova R P, Kucherenko N E, Demchenko I B

出版信息

Radiobiologiia. 1986 Jan-Feb;26(1):88-91.

PMID:3513239
Abstract

X-irradiation of animals with an absolutely lethal dose of 0.21 C/kg increases the incorporation of a methyl group into aminoacyl-tRNA-synthetases the radioactive label being traced in lysine, arginine, and histidine. The isolated protein methyltransferase methylated total preparations of aminoacyl-tRNA-synthetases and lysyl-tRNA-synthetase of rat liver in the in vitro experiments with the use of S-adenosylmethionine-14CH3 as a donor of methyl groups: the radioactive label was traced in arginine, lysine, histidine, aspartic and glutamic acids.

摘要

用绝对致死剂量0.21 C/kg对动物进行X射线照射,会增加甲基掺入氨酰-tRNA合成酶的量,放射性标记可在赖氨酸、精氨酸和组氨酸中检测到。在体外实验中,使用S-腺苷甲硫氨酸-14CH3作为甲基供体,分离出的蛋白质甲基转移酶使大鼠肝脏氨酰-tRNA合成酶和赖氨酰-tRNA合成酶的总制剂发生甲基化:放射性标记可在精氨酸、赖氨酸、组氨酸、天冬氨酸和谷氨酸中检测到。

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