Biochemistry Department, Faculty of Medicine, Universidad Nacional Autónoma de México, Mexico City.
Institute of Biological Research, Universidad Veracruzana, Veracruz, Mexico.
Gac Med Mex. 2021;157(4):343-349. doi: 10.24875/GMM.M21000573.
Sarcoplasmic reticulum Ca ATPases (SERCA) enzymes are essential for intracellular Ca homeostasis. SERCA genes (ATP2A1-3) encode for different functional isoforms of the protein, whose expression or function is altered in several types of cancer, such as gastric and oral, as well as colon, breast, lung, thyroid, liver, and prostate cancer, among others. However, the role played by SERCA pumps in carcinogenesis is unknown.
Techniques such as real-time polymerase chain reaction, optical microscopy, proliferation and cell death assays, as well as bioinformatic analyses were used.
To evaluate the expression levels of the ATP2A2 and ATP2A3 genes in cell lines representative of different subtypes of breast cancer.
The results show that the MDA-MB-231 cell line expresses lower mRNA levels for the ATP2A3 gene in comparison with MCF-7 cells. The use of the phytoestrogen resveratrol induces ATP2A3 expression, decreases proliferation, and induces apoptosis in both cell types.
SERCA expression might function as a tool to differentiate breast cancer subtypes, which have different treatment requirements.
肌浆网 Ca2+-ATP 酶（SERCA）酶对于细胞内 Ca2+稳态至关重要。SERCA 基因（ATP2A1-3）编码蛋白的不同功能同工型，其表达或功能在多种类型的癌症中发生改变，例如胃癌和口腔癌，以及结肠癌、乳腺癌、肺癌、甲状腺癌、肝癌和前列腺癌等。然而，SERCA 泵在癌症发生中的作用尚不清楚。
使用实时聚合酶链反应、光学显微镜、增殖和细胞死亡测定以及生物信息学分析等技术。
评估不同乳腺癌亚型代表细胞系中 ATP2A2 和 ATP2A3 基因的表达水平。
结果表明，与 MCF-7 细胞相比，MDA-MB-231 细胞系中 ATP2A3 基因的 mRNA 水平较低。使用植物雌激素白藜芦醇可诱导两种细胞类型中 ATP2A3 的表达，降低增殖并诱导细胞凋亡。
SERCA 表达可能作为区分具有不同治疗需求的乳腺癌亚型的工具。
