Pegoraro Caterina, Pollet Nicolas, Monsoro-Burq Anne H
Institut Curie, Research division, CNRS UMR3347, INSERM U1021, Centre Universitaire, Orsay F-91 405, France.
Gene Expr Patterns. 2011 Jan-Feb;11(1-2):122-8. doi: 10.1016/j.gep.2010.10.006. Epub 2010 Nov 11.
Calcium-ATPase pumps are critical in most cells, to sequester calcium into intracytoplasmic stores and regulate general calcium signalling. In addition, cell-specific needs for calcium signals have been described and employ a diversity of calcium ATPases in adult tissues and oocytes. A major family of such calcium pumps is ATP2A/SERCA family, for Sarcoplasmic/Endoplasmic Reticulum Calcium ATPases. Although largely studied in adults, the developmental expression of the atp2a/serca genes remains unknown. Here, we provide genome organisation in Xenopuslaevis and tropicalis and phylogeny of atp2a/serca genes in craniates. We detail embryonic expression for the three X. laevis atp2a/serca genes. We found that the three atp2a/serca genes are strongly conserved among vertebrates and display complementary and tissue-specific expression in embryos. These expression patterns present variations when compared to the data reported in adults. Atp2a1/serca1 is expressed as soon as the end of gastrulation in a subset of the myod-positive cells, and later labels prospective slow muscle cells in the superficial part of the somite. In contrast atp2a2/serca2 is found in a larger subset of cells, but is not ubiquitous as reported in adults. Notably, atp2a2/serca2 is prominently expressed in the neural-related tissues, i.e. the neural plate, cement gland, but is excluded from premigratory neural crest. Finally, atp2a3/serca3 expression is restricted to the ectoderm throughout development.
钙 - ATP酶泵在大多数细胞中至关重要，可将钙隔离到胞质内储存库并调节一般的钙信号传导。此外，已经描述了细胞对钙信号的特定需求，并且在成体组织和卵母细胞中使用多种钙ATP酶。这类钙泵的一个主要家族是ATP2A / SERCA家族，即肌浆网/内质网钙ATP酶。尽管在成体中进行了大量研究，但atp2a / serca基因的发育表达仍不清楚。在这里，我们提供了非洲爪蟾和热带爪蟾的基因组组织以及脊椎动物中atp2a / serca基因的系统发育。我们详细描述了非洲爪蟾三个atp2a / serca基因的胚胎表达。我们发现这三个atp2a / serca基因在脊椎动物中高度保守，并在胚胎中表现出互补的组织特异性表达。与成体中报道的数据相比，这些表达模式存在差异。Atp2a1 / serca1在原肠胚形成末期就在一部分肌细胞决定因子阳性细胞中表达，随后标记体节表层的预期慢肌细胞。相比之下，atp2a2 / serca2存在于更大的细胞亚群中，但不像在成体中报道的那样普遍存在。值得注意的是，atp2a2 / serca2在神经相关组织中显著表达，即神经板、黏腺，但不表达于迁移前的神经嵴。最后，atp2a3 / serca3的表达在整个发育过程中都局限于外胚层。
