Facciol Amanda, Marawi Tulip, Syed Erum, Gerlai Robert
Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto M5S 3G5, ON, Canada.
Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga L5L 1C6, ON, Canada.
Pharmacol Biochem Behav. 2022 Mar;214:173342. doi: 10.1016/j.pbb.2022.173342. Epub 2022 Feb 5.
Fetal Alcohol Spectrum Disorder (FASD) is characterized by a variety of morphological, behavioural and cognitive deficits, ranging from mild to severe. Numerous animal models, including the zebrafish, have been employed to better understand the onset, expression and progression of this disorder. Embryonic ethanol-induced deficits in learning and memory, anxiety, social responses and elevated alcohol self-administration have been successfully demonstrated in zebrafish. Studies in zebrafish have also shown the expression of these behavioural deficits depends upon the developmental stage of ethanol exposure, the age of observation, as well as the genotype (strain or population origin) of the tested zebrafish. Here, we investigate how the genotype and age of observation may influence embryonic ethanol-induced alterations in anxiety-like responses in zebrafish. Zebrafish embryos exposed to either 0% or 1% (vol/vol) ethanol at 24hpf were tested in an open tank at one of three stages: larval (6-8 days post fertilization (dpf)), mid-larval (16-18dpf), or juvenile (26-28dpf). Two genotypes were tested in this manner, AB (a quasi-inbred strain) and AB (a mix of AB, TU and TL strains). We found embryonic ethanol induced behavioural changes to significantly differ depending on the genotype and age of observation. For example, significant differences between control and ethanol exposed zebrafish in both genotypes were observed in juvenile zebrafish, but few significant treatment effects were observed in larval zebrafish. Additionally, ethanol appeared to alter anxiety-like behaviours in the AB genotype but did not have as robust of an effect on the AB genotype. Lastly, there were significant behavioural differences between unexposed (control) zebrafish of the two genotypes, suggesting baseline behavioural differences despite a common AB genetic origin.
胎儿酒精谱系障碍（FASD）的特征是存在从轻度到重度的各种形态、行为和认知缺陷。包括斑马鱼在内的众多动物模型已被用于更好地理解这种障碍的发病、表现和进展。在斑马鱼中已成功证明胚胎期乙醇诱导的学习和记忆、焦虑、社交反应缺陷以及酒精自我给药增加。对斑马鱼的研究还表明，这些行为缺陷的表现取决于乙醇暴露的发育阶段、观察年龄以及受试斑马鱼的基因型（品系或种群来源）。在此，我们研究基因型和观察年龄如何影响胚胎期乙醇诱导的斑马鱼焦虑样反应变化。在受精后24小时（hpf）暴露于0%或1%（体积/体积）乙醇的斑马鱼胚胎在三个阶段之一的开放水箱中进行测试：幼体期（受精后6 - 8天（dpf））、中幼体期（16 - 18 dpf）或幼年期（26 - 28 dpf）。以这种方式测试了两种基因型，AB（一种近交系品系）和AB（AB、TU和TL品系的混合）。我们发现胚胎期乙醇诱导的行为变化因基因型和观察年龄而异。例如，在幼年期斑马鱼中观察到两种基因型的对照和乙醇暴露斑马鱼之间存在显著差异，但在幼体期斑马鱼中几乎未观察到显著的处理效应。此外，乙醇似乎改变了AB基因型的焦虑样行为，但对AB基因型的影响没有那么强烈。最后，两种基因型的未暴露（对照）斑马鱼之间存在显著的行为差异，这表明尽管有共同的AB遗传起源，但存在基线行为差异。
