Microbiology and Hygiene Group, Institute of Precision Medicine, Faculty of Medical & Life Sciences, Furtwangen University, Villingen-Schwenningen, Germany.
Organic and Bioorganic Chemistry Lab, Institute of Precision Medicine, Faculty of Medical & Life Sciences, Furtwangen University, Villingen-Schwenningen, Germany.
J Microbiol Methods. 2022 Mar;194:106432. doi: 10.1016/j.mimet.2022.106432. Epub 2022 Feb 5.
Cesium trifluoroacetate (CsTFA) is a gradient medium for isopycnic centrifugation in RNA-based Stable Isotope Probing (RNA-SIP), an important means to link the structure and function of microbial communities. We report a protocol to easily synthesize CsTFA from cesium carbonate (CsCO) and trifluoroacetic acid (TFA) and show that self-synthesized CsTFA performs similarly to commercial CsTFA in the separation of isotopically labelled and unlabelled bacterial RNA.
三氟乙酸铯（CsTFA）是基于 RNA 的稳定同位素探测（RNA-SIP）等密度梯度离心的梯度介质，是连接微生物群落结构和功能的重要手段。本研究从碳酸铯（CsCO）和三氟乙酸（TFA）中轻松合成 CsTFA，并展示了自制 CsTFA 与商用 CsTFA 一样可用于分离同位素标记和未标记的细菌 RNA。
