Banu Sarena, Gaur Namami, Nair Sowmya, Ravikrishnan Tanuja, Khan Shahida, Mani Sandhya, Bharathi Swarna, Mandal Komal, Kuram Naga Anusha, Vuppaladadium Sowmya, Ravi Rowmika, Murthy Ch Lakshmi N, Quoseena Mir, Babu Nukala Sarath, Idris Mohammed M
CSIR-CCMB, Uppal Road, Hyderabad 500007, India.
CSIR-CCMB, Uppal Road, Hyderabad 500007, India.
Genomics. 2022 Mar;114(2):110300. doi: 10.1016/j.ygeno.2022.110300. Epub 2022 Feb 5.
The complex epimorphic regeneration of zebrafish caudal fin tissue is hasty and absolute. This study was executed to understand the role of various genes/proteins involved in the regeneration of zebrafish caudal fin tissue through differential transcriptomics and proteomics analysis. Based on our study 1408 genes and 661 proteins were found differentially regulated in the regenerating caudal fin tissue for having at least 1-log fold change. Interleukin, Solute carrier, Protein arginine methyltransferase, Homeobox, Neurotransmitter and several novel genes were found to be associated with regeneration for its differential regulation during the mechanism. Based on the network and pathway analysis the differentially regulated genes and proteins were found allied with activation of cell proliferation, cell viability, cell survival & cell movement and inactivation of organismal death, morbidity, necrosis, death of embryo & cell death. This study has mapped a detailed insight of the genes/proteins expression associated with the epimorphic regeneration more profoundly.
斑马鱼尾鳍组织复杂的形态发生再生迅速且彻底。本研究旨在通过差异转录组学和蛋白质组学分析，了解参与斑马鱼尾鳍组织再生的各种基因/蛋白质的作用。基于我们的研究，在再生的尾鳍组织中发现1408个基因和661种蛋白质存在差异调节，其变化倍数至少为1-log。白细胞介素、溶质载体、蛋白质精氨酸甲基转移酶、同源框、神经递质和几个新基因在该机制中因其差异调节而被发现与再生相关。基于网络和通路分析，发现差异调节的基因和蛋白质与细胞增殖、细胞活力、细胞存活和细胞运动的激活以及机体死亡、发病、坏死、胚胎死亡和细胞死亡的失活有关。这项研究更深入地详细描绘了与形态发生再生相关的基因/蛋白质表达情况。
