经口 RNAi 介导的蚊虫基因沉默：从实验室到田间。
Oral RNAi for Gene Silencing in Mosquitoes: From the Bench to the Field.
机构信息
Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, Indiana 46617, USA.
Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana 46556, USA.
出版信息
Cold Spring Harb Protoc. 2022 Jul 12;2022(7):Pdb.top107690. doi: 10.1101/pdb.top107690.
RNA interference (RNAi) has played a key role in the field of insect functional genomics, a discipline that has enhanced the study of developmental, evolutionary, physiological, and molecular biological phenomena in a wide variety of insects, including disease vector mosquitoes. Here we introduce a recently optimized RNAi procedure in which adult mosquitoes are fed with a colored sugar bait containing small interfering RNA (siRNA). This procedure effectively and economically leads to gene silencing, is technically straightforward, and has been successfully used to characterize a number of genes in adult mosquitoes. We also discuss how, in addition to laboratory applications, this oral RNAi procedure might one day be used in the field for controlling insect pests.
RNA 干扰（RNAi）在昆虫功能基因组学领域发挥了关键作用，该学科增强了对包括病媒蚊在内的多种昆虫的发育、进化、生理和分子生物学现象的研究。在这里，我们介绍了一种最近优化的 RNAi 程序，该程序使成年蚊子通过吸食含有小干扰 RNA（siRNA）的有色糖饵来实现基因沉默。该程序有效且经济，技术上简单直接，并已成功用于鉴定成年蚊子中的许多基因。我们还讨论了除了实验室应用之外，这种口服 RNAi 程序将来如何在田间用于控制害虫。
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