Bindels R J, Geertsen J A, Van Os C H
Am J Physiol. 1986 Mar;250(3 Pt 2):F470-5. doi: 10.1152/ajprenal.1986.250.3.F470.
Phosphate metabolism was studied in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) by measuring serum phosphate concentrations and 24-h urinary phosphate excretions in rats placed in metabolic cages from 6 to 23 wk of age. Serum phosphate concentrations in SHR were significantly lower than those in WKY at 6, 12, and 20 wk of age. In addition, 24-h urinary phosphate excretion was lower in SHR relative to WKY from 6 through 23 wk of age. The hypophosphaturia in SHR was accompanied with an increase in the maximal transport rate of Na+-dependent phosphate transport in renal brush border membrane vesicles (BBMV) isolated from kidney cortex at 6, 12, and 20 wk of age. The apparent affinity for phosphate did not differ significantly between WKY and SHR at all ages studied. A direct relationship between maximal Na+-dependent phosphate transport rates in BBMV and serum phosphate concentrations was observed in both strains. In SHR, phosphate homeostasis is disturbed from 6 wk of age on.
通过测量6至23周龄置于代谢笼中的自发性高血压大鼠（SHR）和正常血压的Wistar-Kyoto大鼠（WKY）的血清磷酸盐浓度和24小时尿磷酸盐排泄量，研究了磷酸盐代谢。在6周、12周和20周龄时，SHR的血清磷酸盐浓度显著低于WKY。此外，从6周到23周龄，SHR的24小时尿磷酸盐排泄量相对于WKY更低。SHR的低磷尿症伴随着从6周、12周和20周龄的肾皮质分离的肾刷状缘膜囊泡（BBMV）中钠依赖性磷酸盐转运的最大转运速率增加。在所有研究年龄中，WKY和SHR对磷酸盐的表观亲和力没有显著差异。在两个品系中均观察到BBMV中最大钠依赖性磷酸盐转运速率与血清磷酸盐浓度之间存在直接关系。在SHR中，从6周龄起磷酸盐稳态就受到干扰。
