Yu Youlin, You Shuqing, Fan Rengen, Shan Xiangxiang
Department of Hepatobiliary Surgery, Affiliated Hospital of Shaoxing University, Shaoxing 312000, Zhejiang Province, China.
Department of Pathology, Taizhou First People's Hospital, Taizhou 318020, Zhejinag Province, China.
Future Oncol. 2022 Mar;18(8):979-990. doi: 10.2217/fon-2021-0271. Epub 2022 Feb 9.
This study mainly explores how impacts the progression of hepatocellular carcinoma (HCC). Mature miRNA and mRNA expression data along with the clinical data of HCC were provided by The Cancer Genome Atlas to mine differentially expressed miRNAs and mRNAs. Expression levels of and miR-139-3p in HCC were tested through quantitative real-time PCR. How and miR-139-3p impacted HCC cell activities were detected by Transwell, wound healing and cell proliferation approaches. Whether miR-139-3p could bind to was detected by dual-luciferase assay. This investigation found evidently high levels of in both HCC tissue and cells and its marked association with poor prognosis. Overexpression of could significantly promote the behaviors of HCC cells. In addition, poorly expressed miR-139-3p was inversely associated with . Dual-luciferase method also proved the association. The rescue experiment showed that miR-139-3p regulated cell behaviors in HCC through targeting . Highly expressed was mediated by miR-139-3p to modulate cell behaviors in HCC. It is assumed that is a possible target of HCC for cancer therapy purposes.
本研究主要探讨[具体内容]如何影响肝细胞癌（HCC）的进展。癌症基因组图谱（The Cancer Genome Atlas）提供了HCC的成熟miRNA和mRNA表达数据以及临床数据，以挖掘差异表达的miRNA和mRNA。通过定量实时PCR检测HCC中[具体内容]和miR-139-3p的表达水平。采用Transwell、伤口愈合和细胞增殖方法检测[具体内容]和miR-139-3p如何影响HCC细胞活性。通过双荧光素酶测定法检测miR-139-3p是否能与[具体内容]结合。本研究发现HCC组织和细胞中[具体内容]水平明显较高，且与预后不良显著相关。[具体内容]的过表达可显著促进HCC细胞的行为。此外，miR-139-3p低表达与[具体内容]呈负相关。双荧光素酶法也证实了这种关联。挽救实验表明，miR-139-3p通过靶向[具体内容]调节HCC细胞行为。miR-139-3p介导高表达的[具体内容]调节HCC细胞行为。推测[具体内容]可能是HCC癌症治疗的一个靶点。
