Department of Periodontology, Nihon University School of Dentistry at Matsudo, Matsudo, Japan.
Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Japan.
Cell Adh Migr. 2022 Dec;16(1):13-24. doi: 10.1080/19336918.2022.2029237.
Laminin 5, type 4 collagen, and α6β4 integrin contribute to the formation of hemidesmosomes in the epithelia of periodontal tissues, which is critical for the development and maintenance of the dentogingival junction. As it is not known whether TNF-α alters the composition of the epithelial pericellular matrix, human gingival epithelial cells were cultured in the presence or absence of TNF-α. Treatment with TNF-α accelerated epithelial cell migration and closure of in vitro wounds. These data indicate unexpectedly, that TNF-α promotes the formation of the pericellular matrix around epithelial cells and enhances adhesion of epithelial cells to the underlying matrix, properties which are important for cell migration and the integrity of the dentogingival junction.
层粘连蛋白 5、IV 型胶原蛋白和α6β4 整合素有助于牙周组织上皮形成半桥粒，这对于牙龈结合处的发育和维持至关重要。由于尚不清楚 TNF-α 是否会改变上皮细胞周细胞基质的组成，因此将人牙龈上皮细胞在存在或不存在 TNF-α的情况下进行培养。TNF-α 处理可加速上皮细胞迁移并封闭体外伤口。这些数据出人意料地表明，TNF-α促进了围绕上皮细胞的细胞周基质的形成，并增强了上皮细胞与基底基质的黏附，这些特性对于细胞迁移和牙龈结合处的完整性很重要。
