Morgan G, Linch D C, Knott L T, Davies E G, Sieff C, Chessells J M, Hale G, Waldmann H, Levinsky R J
Br J Haematol. 1986 Mar;62(3):421-30. doi: 10.1111/j.1365-2141.1986.tb02953.x.
Six patients with severe combined immunodeficiency were transplanted with bone marrow from their HLA haploidentical parents. T-lymphocytes were removed by complement mediated lysis with a monoclonal antibody (CAMPATH-I) followed by rosetting with sheep erythrocytes. The patients were pre-conditioned with marrow ablative chemotherapy, using busulphan and cyclophosphamide. There was graft take in five patients, with evidence of transient acute graft-versus-host disease only. Three patients are alive and well greater than 5 months after transplant, and one who is 12 months post graft has full reconstitution of cell mediated and humoral immunity, including specific antibody response to immunization. Transplantation across major histocompatibility barriers can be successfully achieved using this method of treatment of donor marrow.
6例重症联合免疫缺陷患者接受了来自其HLA单倍型相同父母的骨髓移植。先用单克隆抗体（CAMPATH-1）通过补体介导的细胞溶解法去除T淋巴细胞，随后用绵羊红细胞进行花环试验。患者接受了白消安和环磷酰胺的骨髓清除性化疗预处理。5例患者出现移植物植入，仅表现为短暂的急性移植物抗宿主病。3例患者移植后5个月以上存活且状况良好，1例移植后12个月的患者细胞介导免疫和体液免疫完全重建，包括对免疫接种的特异性抗体反应。使用这种供体骨髓治疗方法可以成功实现跨越主要组织相容性屏障的移植。
