Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, 11549, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Curr Pharm Biotechnol. 2022;23(14):1758-1771. doi: 10.2174/1389201023666220209125306.
Medicinal plants are recognized to have a wide range of important biological activities, Spergula fallax L. is a well-known plant while phytochemical and biological activities screening of the species are rare.
This study aims to evaluate the antioxidant, anticáncer, antimicrobial, and antiviral activities of the methanol extract (ME) of the aerial parts of Spergula fallax L. and its successive fractions; n-hexane (HF), ethyl acetate (EAF), and n-butanol (BF) fractions and identify their biologically active metabolites.
Ultra-performance liquid chromatography (UPLC) coupled to quadrupole high-resolution time-of-flight mass spectrometry (QTOF-MS) was utilized to establish the metabolic profile of Sergula fallax L. Total phenol and flavonoids content were determined colorimetrically using Folin Ciocalteu and aluminum chloride reagents, respectively. 1,2-diphenyl-1-picrylhydrazyl (DPPH) was utilized for estimation of antioxidant activity, cytotoxic activity was determined on Hep-G2, HCT- 116, and MCF-7 cell lines, and agar diffusion method was used for screening of antimicrobial activity while cytopathic effect inhibition assay was utilized for antiviral assay.
Twenty-eight metabolites were identified, flavonoids were predominated (26), it was characterized by the presence of four main aglycones (Apigenin, luteolin, quercetin and kaempferol) with their O and/or C-glycosides based on its MS fragments. ME and EAF significantly scavenged DPPH in a dose-dependent manner with IC 16.99 and 18.74 μg.mL, respectively. Among the tested samples, only ME and EAF showed significant cytotoxic activity against the tested cell lines with IC ranging from 5.541- 9.509 μg.mL. All tested samples significantly inhibited the growth of the tested bacterial strains with inhibition zone diameters ranging from 11.2- 26.4 mm and showed a wide range of antiviral activity.
Generally, S. fallax is potentially bioactive as evident by antioxidant, cytotoxic, antibacterial, and antiviral assays. These activities are correlated with its identified metabolites. The results proved that S. fallax has promising medicinal activities and would be examined in future clinical trials for herbal therapy.
药用植物被认为具有广泛的重要生物活性，Spergula fallax L. 是一种众所周知的植物，而对该物种的植物化学和生物活性筛选却很少。
本研究旨在评估 Spergula fallax L. 地上部分甲醇提取物（ME）及其连续部分；正己烷（HF）、乙酸乙酯（EAF）和正丁醇（BF）部分的抗氧化、抗癌、抗菌和抗病毒活性，并鉴定其具有生物活性的代谢产物。
利用超高效液相色谱（UPLC）与四极杆高分辨率飞行时间质谱（QTOF-MS）联用建立 Spergula fallax L. 的代谢谱。分别使用 Folin Ciocalteu 和三氯化铝试剂比色法测定总酚和类黄酮含量。利用 1,2-二苯基-1-苦基肼（DPPH）测定抗氧化活性，在 Hep-G2、HCT-116 和 MCF-7 细胞系上测定细胞毒性，琼脂扩散法筛选抗菌活性，细胞病变抑制试验测定抗病毒活性。
鉴定出 28 种代谢产物，以类黄酮为主（26 种），其特点是存在四个主要的苷元（芹菜素、木犀草素、槲皮素和山奈酚）及其基于 MS 片段的 O 和/或 C-糖苷。ME 和 EAF 以剂量依赖的方式显著清除 DPPH，IC50 分别为 16.99 和 18.74 μg.mL。在所测试的样品中，只有 ME 和 EAF 对测试的细胞系表现出显著的细胞毒性，IC 范围为 5.541-9.509 μg.mL。所有测试样品均显著抑制测试的细菌菌株的生长，抑菌圈直径范围为 11.2-26.4 mm，并表现出广泛的抗病毒活性。
一般来说，S. fallax 具有抗氧化、细胞毒性、抗菌和抗病毒作用，这表明它具有潜在的生物活性。这些活性与鉴定出的代谢产物有关。结果证明 S. fallax 具有有前途的药用活性，并将在未来的草药治疗临床试验中进行检验。
Zotero 插件