Department of Chemical and Life Sciences, Qurtuba University of Science and Information Technology, Peshawar, Pakistan.
Centre for Plant Sciences and Biodiversity, University of Swat, Charbagh, Pakistan.
Sci Rep. 2024 Mar 7;14(1):5627. doi: 10.1038/s41598-024-55905-w.
Plant extracts are actively being used worldwide due to the presence of biologically active constituents helping in the preservation of food, and to aid against various diseases owing to their antimicrobial and antioxidant potential. The present research work was carried out to investigate the phytochemical constituents, antimicrobial activity, and antioxidant activity of different extracted samples of Euphorbia parviflora. Anti-microbial studies were carried out by Agar well diffusion while the DPPH method was employed for investigating anti-oxidant activity. Three samples from methanol, chloroform, and ethyl acetate extract were tested against five different bacterial strains comprising two species from Gram-negative bacteria i.e., Staphylococcus aureus and Bacillus subtilis and three species from Gram-positive bacteria i.e. Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia along two fungal strains i.e. Candida albicans and Aspergillus niger. The results of the qualitative phytochemical analysis showed that methanolic, chloroformic, and ethylacetate extract of Euphorbia parviflora consist of alkaloids, reducing sugars, flavonoids, terpenoids, tannins, and saponins. The total phenol and flavonoid content of E. parviflora showed that the methanolic extract of E. parviflora had a significantly higher total phenolic content (53.73 ± 0.30 mg of GAE/g) and flavonoid content (44.62 ± 0.38 mg of than other extracts. The content of total phenolic and flavonoids was more in methanolic extract as compared to other extracts of E. prolifera. The HPLC analysis showed that in the chloroform extract of E. parviflora Cinnamic acid (4.32 ± 2.89 mg/g) was dominant, in methanol extract quercetin (3.42 ± 2.89 mg/g) was dominant and in ethyl acetate extract of E. parviflora catechin (4.44 ± 2.89 mg/g) was found dominant. The antimicrobial activity revealed that amongst all the extracts the highest antibacterial activity was shown by methanolic extract against B. subtilis and Staphylococcus aureus as compared to the other extracts. The antioxidant activity revealed that methanolic extract of E. parviflora demonstrated higher antioxidant activity (82.42 ± 0.02) followed by chloroform extract (76.48 ± 0.08) at 150 µg/mL. The aim of this study was primarily to evaluate the potential of this plant as a reliable source of antimicrobials and antioxidants that may be used for the treatment of various infectious diseases in the future. The study provides evidence that this plant can act as a reliable source of antimicrobial and antioxidant agents and might be used against several infectious diseases.
由于含有生物活性成分，植物提取物在全球范围内被广泛应用，有助于保持食品的新鲜度，并因其具有抗菌和抗氧化潜力而有助于预防各种疾病。本研究旨在调查小飞扬草的不同提取样品的植物化学组成、抗菌活性和抗氧化活性。采用琼脂孔扩散法进行抗菌研究，采用 DPPH 法研究抗氧化活性。对甲醇、氯仿和乙酸乙酯三种提取物进行了测试，共测试了五种不同的细菌菌株，包括两种革兰氏阴性菌（金黄色葡萄球菌和枯草芽孢杆菌）和三种革兰氏阳性菌（大肠杆菌、铜绿假单胞菌和肺炎克雷伯菌）以及两种真菌（白色念珠菌和黑曲霉）。定性植物化学分析结果表明，小飞扬草的甲醇、氯仿和乙酸乙酯提取物含有生物碱、还原糖、类黄酮、三萜、单宁和皂苷。小飞扬草的总酚和类黄酮含量表明，小飞扬草的甲醇提取物总酚含量（53.73±0.30 mg GAE/g）和类黄酮含量（44.62±0.38 mg）均显著高于其他提取物。小飞扬草的总酚和类黄酮含量均高于其他提取物。HPLC 分析表明，小飞扬草的氯仿提取物中肉桂酸（4.32±2.89 mg/g）含量较高，甲醇提取物中槲皮素（3.42±2.89 mg/g）含量较高，乙酸乙酯提取物中儿茶素（4.44±2.89 mg/g）含量较高。抗菌活性表明，在所有提取物中，甲醇提取物对枯草芽孢杆菌和金黄色葡萄球菌的抗菌活性最高，高于其他提取物。抗氧化活性表明，小飞扬草的甲醇提取物表现出较高的抗氧化活性（82.42±0.02），高于氯仿提取物（76.48±0.08），在 150 μg/mL 时。本研究的主要目的是评估这种植物作为可靠的抗菌和抗氧化剂来源的潜力，这种植物可能用于未来治疗各种传染病。该研究表明，这种植物可以作为一种可靠的抗菌和抗氧化剂来源，并可能用于治疗多种传染病。
