Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
Biology Department, College of Science, King Khalid University, Abha, Saudi Arabia.
Microsc Res Tech. 2022 Jun;85(6):2192-2205. doi: 10.1002/jemt.24076. Epub 2022 Feb 10.
The current investigation described the ultrastructural and histological features of the lingual papillary system in 14 adult Rhinopoma hardwickii using scanning electron and light microscopy, and immunohistochemical staining of keratin. Three types of lingual papillae were recognized; one filiform mechanical and two gustatory (fungiform and circumvallate) of three directions: posterior, posteromedian, and median. Five filiform papillae subtypes were observed in four regions. The lingual tip had numerous giant filiform papillae. The lingual root was divided into two regions, an anterior papillary and a posterior non-papillary region that had only three circumvallate papillae in a triangular pattern. Histologically, the papillae exhibited detached thick keratin that spread over the dorsal epithelial surface. The dorsal surface of the root had stratified keratinized circumvallate papillae. Concerning the pan-cytokeratin (CK) antibody staining, moderate CK expression was observed in the superficial corneal layer of the apical lingual mucosa. While the superficial corneal cell layer in both body and root exhibited increasing of CK antibody. Morphometrically, there was a significantly increased, region-specific number of triangular, pointed filiform papillae, which were relatively increased in length and width. However, the round conical blunt filiform papillae and fungiform papillae were sparse. We concluded that our results showed distinguished adaptations in the lingual structure and its different papillae established on the nutritional and energetic demand of this bat species. This lingual adaptation is summarized in number, position, taste buds, direction, ultrastructural features, and histological that concerned with its ability in eating during the night flight. RESEARCH HIGHLIGHTS: Three types of lingual papillae; one mechanical (filiform) and two gustatory (fungiform and circumvallate) in which there were three circumvallate papillae on the posterior part of the lingual root in a triangular arrangement with an irregular surface, but the filiform papillae had five subtypes arranged in four regions. The lingual root was divided into two regions, an anterior papillary region and a posterior non-papillary region that only had three circumvallate papillae. Concerning staining with a pan-cytokeratin (CK) antibody, moderate CK expression was observed in the superficial corneal layer of the lingual mucosa of the tongue apex while the superficial corneal cell layer of the body and root exhibited increased expression of the CK antibody compared to the lingual apex.
本研究采用扫描电子显微镜和组织学观察，以及角蛋白免疫组化染色，描述了 14 只成年 hardwicke 氏攀鲈的舌乳头状系统的超微结构和组织学特征。共识别出 3 种舌乳头：1 种丝状机械性和 2 种味觉性（菌状和轮廓乳头），方向为后、后中、中和正中。在 4 个区域观察到 5 种丝状乳头亚型。舌尖有许多巨大的丝状乳头。舌根部分为前乳头状区和后非乳头状区，在后非乳头状区只有 3 个呈三角形排列的轮廓乳头。组织学上，乳头有分离的厚角蛋白，分布在背侧上皮表面。根部的背侧表面有分层的角化轮廓乳头。关于广谱细胞角蛋白（CK）抗体染色，在舌尖舌黏膜的浅层角膜层观察到中等 CK 表达。而在舌体和舌根的浅层角膜细胞层，CK 抗体表达增加。形态计量学上，三角形、尖形丝状乳头的数量明显增加，且长度和宽度相对增加。然而，圆形圆锥形钝丝状乳头和菌状乳头则较少。我们得出结论，我们的结果表明，硬骨舌形攀鲈的舌结构及其不同的乳头存在明显的适应性，这与该蝙蝠物种的营养和能量需求有关。这种舌部的适应性体现在数量、位置、味蕾、方向、超微结构特征和组织学方面，与它在夜间飞行中进食的能力有关。研究亮点：三种舌乳头；一种机械性（丝状）和两种味觉性（菌状和轮廓乳头），其中舌根的后部分布有 3 个呈三角形排列的、表面不规则的轮廓乳头，而丝状乳头有 5 种亚型，分布在 4 个区域。舌根部分为两个区域，一个是前乳头状区域，另一个是后非乳头状区域，该区域只有 3 个轮廓乳头。关于广谱细胞角蛋白（CK）抗体染色，舌尖舌黏膜浅层角膜层观察到中等 CK 表达，而舌体和舌根浅层角膜细胞层 CK 抗体表达增加。
