Feng Qiuying, Liu Xue, Yang Linlin, Fu Zeyuan, Xu Qijiang
Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin 150040, Heilongjiang, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Jan 25;38(1):275-286. doi: 10.13345/j.cjb.210123.
This study aims to investigate the molecular mechanism of the transcription factor MYB10, which is involved in anthocyanin biosynthesis, in different colors of L. fruitification. Rapid amplification of cDNA ends (RACE) was used to clone the genes from L. (), L. (), and L. (), respectively. Phylogenetic analysis showed that and were evolutionarily homologous. Real-time quantitative PCR (RT-qPCR) showed that the expression of in the fruits of L. was higher than that of L. and much higher than that of L. The expression of and increased at first and then decreased as the fruit diameter increased and the fruit color deepened (the maximum expression level was reached at 75% of the fruit color change), while the expression level of was very low. Overexpression of and in resulted in purple petioles and leaves, whereas overexpression of resulted in no significant color changes. This indicates that gene plays an important role in the coloration of L. fruit.
本研究旨在探究参与花青素生物合成的转录因子MYB10在不同颜色枸杞果实形成过程中的分子机制。分别采用cDNA末端快速扩增（RACE）技术从宁夏枸杞、黑果枸杞和红果枸杞中克隆相关基因。系统发育分析表明宁夏枸杞和黑果枸杞在进化上具有同源性。实时定量PCR（RT-qPCR）结果显示，MYB10在宁夏枸杞果实中的表达量高于黑果枸杞，且远高于红果枸杞。随着果实直径增加和果实颜色加深，MYB10和PAP1的表达量先升高后降低（在果实颜色变化75%时达到最高表达水平），而MYB113的表达量很低。在烟草中过表达MYB10和PAP1导致叶柄和叶片呈紫色，而过表达MYB113则未引起显著颜色变化。这表明MYB10基因在枸杞果实着色过程中起重要作用。
