Terano A, Hiraishi H, Ota S, Sugimoto T
Digestion. 1986;33(4):206-10. doi: 10.1159/000199296.
Aim of this study was to investigate the effect of geranylgeranylacetone (GGA) on mucus synthesis and secretion in rat gastric cultured cells, and their relationship to prostaglandin (PG) synthesis. Rate of mucus synthesis was estimated by incorporation of 3H-glucosamine into the cultured cells. Release of 3H-glucosamine from the cells, which were preincubated in the medium containing the radioactive isotope, into the culture media was measured for the evaluation of mucus secretion. PG production by the cultured cells was measured by radioimmunoassay. GGA increased glycoprotein synthesis in a dose-dependent manner (p less than 0.01). Secretion of mucus from cultured cells was also significantly enhanced by GGA. GGA did not significantly increase PG (E2 and I2) production. These results indicate that GGA has the ability to stimulate mucus production by the gastric epithelial cells, and this action may play an important role in protective effect of GGA. It is, however, unlikely that this effect of GGA is mediated by endogenous PGs.
本研究旨在探讨香叶基香叶基丙酮（GGA）对大鼠胃培养细胞黏液合成与分泌的影响，以及它们与前列腺素（PG）合成的关系。通过将3H-葡萄糖胺掺入培养细胞中来估计黏液合成速率。测量在含有放射性同位素的培养基中预孵育的细胞中3H-葡萄糖胺释放到培养基中的情况，以评估黏液分泌。通过放射免疫测定法测量培养细胞产生的PG。GGA以剂量依赖性方式增加糖蛋白合成（p小于0.01）。GGA也显著增强了培养细胞的黏液分泌。GGA没有显著增加PG（E2和I2）的产生。这些结果表明，GGA具有刺激胃上皮细胞产生黏液的能力，并且这种作用可能在GGA的保护作用中起重要作用。然而，GGA的这种作用不太可能由内源性PG介导。
