Bird Lisa, Miles Melina, Quade Adam, Spafford Helen
NSW Department of Primary Industries, Tamworth Agricultural Institute, Calala, New South Wales, Australia.
Queensland Department of Agriculture and Fisheries, Toowoomba, Queensland, Australia.
PLoS One. 2022 Feb 10;17(2):e0263677. doi: 10.1371/journal.pone.0263677. eCollection 2022.
Spodoptera frugiperda (J.E. Smith) is a highly invasive noctuid pest first reported in northern Australia during early 2020. To document current status of resistance in S. frugiperda in Australia, insecticide toxicity was tested in field populations collected during the first year of establishment, between March 2020 and March 2021. Dose-response was measured by larval bioassay in 11 populations of S. frugiperda and a susceptible laboratory strain of Helicoverpa armigera. Emamectin benzoate was the most efficacious insecticide (LC50 0.023μg/ml) followed by chlorantraniliprole (LC50 0.055μg/ml), spinetoram (LC50 0.098μg/ml), spinosad (LC50 0.526μg/ml), and methoxyfenozide (1.413μg/ml). Indoxacarb was the least toxic selective insecticide on S. frugiperda (LC50 3.789μg/ml). Emamectin benzoate, chlorantraniliprole and methoxyfenozide were 2- to 7-fold less toxic on S. frugiperda compared with H. armigera while spinosyns were equally toxic on both species. Indoxacarb was 28-fold less toxic on S. frugiperda compared with H. armigera. There was decreased sensitivity to Group 1 insecticides and synthetic pyrethroids in S. frugiperda compared with H. armigera: toxicity was reduced up to 11-fold for methomyl, 56 to 199-fold for cyhalothrin, and 44 to 132-fold for alpha cypermethrin. Synergism bioassays with metabolic inhibitors suggest involvement of mixed function oxidase in pyrethroid resistance. Recommended diagnostic doses for emamectin benzoate, chlorantraniliprole, spinetoram, spinosad, methoxyfenozide and indoxacarb are 0.19, 1.0, 0.75, 6, 12 and 48μg/μl, respectively.
草地贪夜蛾（J.E. 史密斯）是一种极具入侵性的夜蛾科害虫，2020年初首次在澳大利亚北部被发现。为记录澳大利亚草地贪夜蛾的抗药性现状，于2020年3月至2021年3月该害虫在澳大利亚首次定殖的第一年期间，对采集的田间种群进行了杀虫剂毒性测试。通过幼虫生物测定法测定了11个草地贪夜蛾种群和一种敏感的棉铃虫实验室品系的剂量反应。甲氨基阿维菌素苯甲酸盐是最有效的杀虫剂（LC50为0.023μg/ml），其次是氯虫苯甲酰胺（LC50为0.055μg/ml）、多杀霉素（LC50为0.098μg/ml）、多杀菌素（LC50为0.526μg/ml）和甲氧虫酰肼（1.413μg/ml）/ml）。茚虫威是对草地贪夜蛾毒性最低的选择性杀虫剂（LC50为3.789μg/ml）。与棉铃虫相比，甲氨基阿维菌素苯甲酸盐、氯虫苯甲酰胺和甲氧虫酰肼对草地贪夜蛾的毒性低2至7倍，而多杀菌素对两种害虫的毒性相同。与棉铃虫相比，茚虫威对草地贪夜蛾的毒性低28倍。与棉铃虫相比，草地贪夜蛾对第1组杀虫剂和拟除虫菊酯类杀虫剂的敏感性降低：灭多威的毒性降低了11倍，氯氟氰菊酯的毒性降低了56至199倍，高效氯氰菊酯的毒性降低了44至132倍。与代谢抑制剂的增效生物测定表明，多功能氧化酶参与了拟除虫菊酯抗性。甲氨基阿维菌素苯甲酸盐、氯虫苯甲酰胺、多杀霉素、多杀菌素、甲氧虫酰肼和茚虫威的推荐诊断剂量分别为0.19、1.0、0.75、6、12和48μg/μl。
