Williams K M, Sacci J B, Anthony R L
Infect Immun. 1986 Apr;52(1):36-44. doi: 10.1128/iai.52.1.36-44.1986.
A flow cytometric assay was developed to quantitate the binding kinetics of Leishmania mexicana subsp. mexicana promastigotes to murine peritoneal macrophages and to determine if selected membrane-specific monoclonal antibodies would exert an effect on the binding process. A total of three monoclonal antibodies, all reactive with a similar 42-kilodalton surface membrane component by Western blot analysis, enhanced parasite-macrophage binding at levels greater than 45%. An additional three monoclonal antibodies that identified low-molecular-weight antigens of the promastigote (15 to 20 kilodaltons) had no effect on the binding process. Of these antibodies, however, one did appear to inhibit the internalization of the parasites after it attached to the macrophage membrane.
开发了一种流式细胞术检测方法，用于定量墨西哥利什曼原虫墨西哥亚种前鞭毛体与小鼠腹腔巨噬细胞的结合动力学，并确定所选的膜特异性单克隆抗体是否会对结合过程产生影响。通过蛋白质印迹分析，共有三种单克隆抗体均与一种相似的42千道尔顿表面膜成分发生反应，它们使寄生虫与巨噬细胞的结合增强了45%以上。另外三种识别前鞭毛体低分子量抗原（15至20千道尔顿）的单克隆抗体对结合过程没有影响。然而，在这些抗体中，有一种抗体似乎在寄生虫附着于巨噬细胞膜后抑制其内化。
