• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过DEAE亲和蓝胶色谱法从腹水原液中一步纯化小鼠单克隆抗体。

One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography.

作者信息

Bruck C, Portetelle D, Glineur C, Bollen A

出版信息

J Immunol Methods. 1982 Sep 30;53(3):313-9. doi: 10.1016/0022-1759(82)90178-8.

DOI:10.1016/0022-1759(82)90178-8
PMID:7142712
Abstract

Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities.

摘要

单克隆抗体可通过在DEAE琼脂糖蓝柱上进行色谱分离，直接从腹水液中纯化。确定了回收不含污染性蛋白酶和核酸酶活性的免疫球蛋白的最佳条件。

相似文献

1
One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography.通过DEAE亲和蓝胶色谱法从腹水原液中一步纯化小鼠单克隆抗体。
J Immunol Methods. 1982 Sep 30;53(3):313-9. doi: 10.1016/0022-1759(82)90178-8.
2
Purification of IgM monoclonal antibody from murine ascitic fluid by a two-step column chromatography procedure.通过两步柱色谱法从鼠腹水纯化IgM单克隆抗体。
Immunol Lett. 1987 Jan;14(2):159-65. doi: 10.1016/0165-2478(87)90096-4.
3
Purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel Blue chromatography.
Methods Enzymol. 1986;121:587-96. doi: 10.1016/0076-6879(86)21058-7.
4
One-step purification of mouse monoclonal antibodies from ascites fluid by hydroxylapatite chromatography.通过羟基磷灰石色谱法从腹水原液中一步纯化小鼠单克隆抗体。
J Immunol Methods. 1985 Jan 21;76(1):157-69. doi: 10.1016/0022-1759(85)90488-0.
5
Purification of ascitic fluid-derived murine monoclonal antibodies by anion-exchange and size-exclusion high-performance liquid chromatography.通过阴离子交换和尺寸排阻高效液相色谱法纯化腹水来源的鼠单克隆抗体。
J Chromatogr. 1988 Sep 9;430(2):329-39. doi: 10.1016/s0378-4347(00)83168-0.
6
Purification of monoclonal antibodies from ascites using ABx liquid chromatography column.使用ABx液相色谱柱从腹水中纯化单克隆抗体。
J Immunol Methods. 1987 Sep 24;102(2):227-31. doi: 10.1016/0022-1759(87)90081-0.
7
FPLC purification of mouse monoclonal antibodies from ascitic fluid using blue DEAE and thiophilic sorbents.使用蓝色葡聚糖二乙氨基乙基纤维素和嗜硫吸附剂从腹水液中通过快速蛋白质液相色谱法纯化小鼠单克隆抗体。
J Immunol Methods. 1991 Jan 24;136(1):103-9. doi: 10.1016/0022-1759(91)90255-e.
8
[Isolation of monoclonal antibodies from mouse ascitic fluid using chromatography on "blue" DEAE cellulose].
Cesk Farm. 1989 Jul;38(6):276-8.
9
Purification of monoclonal antibodies from ascitic fluid using preparative electrophoresis.使用制备电泳从腹水液中纯化单克隆抗体。
J Chromatogr A. 1998 Dec 11;827(2):329-35. doi: 10.1016/s0021-9673(98)00847-4.
10
Tandem purification of IgM monoclonal antibodies from mouse ascites fluids by anion-exchange and gel fast protein liquid chromatography.通过阴离子交换和凝胶快速蛋白质液相色谱法从小鼠腹水中串联纯化IgM单克隆抗体。
J Chromatogr. 1986 Feb 28;354:425-33. doi: 10.1016/s0021-9673(01)87043-6.

引用本文的文献

1
Monoclonal IgA Antibodies for Aflatoxin Immunoassays.用于黄曲霉毒素免疫测定的单克隆IgA抗体。
Toxins (Basel). 2016 May 12;8(5):148. doi: 10.3390/toxins8050148.
2
S2 episome of maize mitochondria encodes a 130-kilodalton protein found in male sterile and fertile plants.玉米线粒体 S2 质体编码一个 130 千道尔顿的蛋白质，该蛋白存在于雄性不育和可育植物中。
Proc Natl Acad Sci U S A. 1987 Nov;84(22):7861-5. doi: 10.1073/pnas.84.22.7861.
3
Preparation and purification of F(ab')(2) fragment from anti hepatoma mouse IgG(1) mAb.从抗肝癌小鼠IgG(1)单克隆抗体中制备和纯化F(ab')(2)片段
World J Gastroenterol. 1999 Dec;5(6):522-524. doi: 10.3748/wjg.v5.i6.522.
4
Microinjection of anti-coilin antibodies affects the structure of coiled bodies.显微注射抗卷曲螺旋蛋白抗体影响卷曲小体的结构。
J Cell Biol. 1998 Aug 24;142(4):899-912. doi: 10.1083/jcb.142.4.899.
5
Possible role for a human adenovirus in the pathogenesis of celiac disease.人腺病毒在乳糜泻发病机制中的可能作用。
J Exp Med. 1984 Nov 1;160(5):1544-57. doi: 10.1084/jem.160.5.1544.
6
Interaction of monoclonal antibodies with pertussis toxin and its subunits.单克隆抗体与百日咳毒素及其亚基的相互作用。
Infect Immun. 1984 Oct;46(1):195-201. doi: 10.1128/iai.46.1.195-201.1984.
7
Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin.针对劳氏肉瘤病毒pp60src的单克隆抗体可与源自禽类和哺乳动物的具有酶活性的细胞pp60src发生反应。
J Virol. 1984 Aug;51(2):272-82. doi: 10.1128/JVI.51.2.272-282.1984.
8
Monoclonal antibody against pertussis toxin: effect on toxin activity and pertussis infections.抗百日咳毒素单克隆抗体：对毒素活性和百日咳感染的影响。
Infect Immun. 1984 Nov;46(2):422-8. doi: 10.1128/iai.46.2.422-428.1984.
9
Binding to cells of virosomes containing herpes simplex virus type 1 glycoproteins and evidence for fusion.与含有单纯疱疹病毒1型糖蛋白的病毒体的细胞结合及融合证据。
J Virol. 1984 Oct;52(1):238-47. doi: 10.1128/JVI.52.1.238-247.1984.
10
Antibodies to a synthetic oligopeptide that react with herpes simplex virus type 1 and 2 glycoprotein C.与单纯疱疹病毒1型和2型糖蛋白C发生反应的针对合成寡肽的抗体。
J Virol. 1984 Aug;51(2):430-6. doi: 10.1128/JVI.51.2.430-436.1984.