印度尼西亚齐-尼氏染色法诊断担子菌病面临的挑战：一则独特病例报告
Challenge of Ziehl-Neelsen stain for Basidiobolomycosis diagnosis in Indonesia: A unique case report.
作者信息
Octora Metta, Kawilarang Arthur Pohan, Endraswari Pepy Dwi
机构信息
Study Program of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Faculty of Medicine, Universitas Mataram, Mataram, Indonesia.
出版信息
Ann Med Surg (Lond). 2022 Jan 25;74:103278. doi: 10.1016/j.amsu.2022.103278. eCollection 2022 Feb.
BACKGROUND
Basidiobolomycosis is a rare fungal infection in Indonesia, so alternative investigations are needed to make a diagnosis.
CASE PRESENTATION
A 24-year-old male patient, Javanese, presented with Basidiobolomycosis. The patient had a lump on the right arm and face 2 years ago, and the tumor progressively grew up and extended to the right upper arm, neck, and face since 1 month ago. This paper discussed the challenge of Ziehl-Neelsen (ZN) stain as a diagnostic tool for Basidiobolomycosis that is a rare case in the world, especially in Indonesia.
DISCUSSION
Acid-fast stain has never been used to diagnose fungal cases in general, but it could describe the hyphae and Splendore-Hoeppli very clearly compared to the standard hematoxylin and eosin (HE) stains.
CONCLUSION
ZN can be used as an alternative diagnosis of Basidiobolomycosis in a low-resource setting.
背景
担子菌病在印度尼西亚是一种罕见的真菌感染，因此需要其他检查来进行诊断。
病例报告
一名24岁的爪哇男性患者患有担子菌病。该患者两年前右臂和面部出现肿块，自1个月前起肿瘤逐渐长大并蔓延至右上臂、颈部和面部。本文讨论了萋-尼（ZN）染色作为担子菌病诊断工具所面临的挑战，担子菌病在世界范围内尤其是在印度尼西亚是一种罕见病例。
讨论
抗酸染色一般从未用于诊断真菌病例，但与标准苏木精和伊红（HE）染色相比，它能非常清晰地显示菌丝和Splendore-Hoeppli现象。
结论
在资源匮乏的环境中，ZN染色可作为担子菌病的一种替代诊断方法。
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