应用流式细胞术鉴定沙门氏菌的菌系。
Identifying Bacterial Lineages in Salmonella by Flow Cytometry.
机构信息
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain.
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Seville, Spain.
出版信息
EcoSal Plus. 2022 Dec 15;10(1):eESP00182021. doi: 10.1128/ecosalplus.ESP-0018-2021. Epub 2022 Feb 4.
Abstract
Advances in technologies that permit high-resolution analysis of events in single cells have revealed that phenotypic heterogeneity is a widespread phenomenon in bacteria. Flow cytometry has the potential to describe the distribution of cellular properties within a population of bacterial cells and has yielded invaluable information about the ability of isogenic cells to diversify into phenotypic subpopulations. This review will discuss several single-cell approaches that have recently been applied to define phenotypic heterogeneity in populations of Salmonella enterica.
摘要
技术的进步使得对单细胞事件进行高分辨率分析成为可能，这揭示了表型异质性是细菌中普遍存在的现象。流式细胞术有可能描述细菌细胞群体中细胞特性的分布，并为同基因细胞分化为表型亚群的能力提供了宝贵的信息。本文将讨论最近应用于定义沙门氏菌属群体中表型异质性的几种单细胞方法。
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