Integrated Bioprocessing Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, 603 203, India; Department of Biotechnology, Faculty of Engineering, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, 641 021, India.
Integrated Bioprocessing Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, 603 203, India; Department of Biochemistry, FASH, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, 641 021, India.
Environ Res. 2022 Jun;209:112926. doi: 10.1016/j.envres.2022.112926. Epub 2022 Feb 8.
Remediation of persistent polycyclic aromatic hydrocarbons (PAHs) contaminated soil has become a major challenge in recent years. Further, conventional application of bioaugmentation strategies for PAHs remediation require continuous supply of microbial specific nutrients, which makes these processes less feasible. Hence, the present study focused on PAHs remediation using surfactants along with wood assisted fungal system in a microcosm set up. In this study, in absence of surfactants, a saturation in PAHs degradation was noted in bioaugmentation with wood assisted fungal system (BAW) with 61 ± 1.25% degradation, followed by bioaugmentation with free fungi system (BAF) (54 ± 0.46%). However, with addition of 1500 mg/L of surface-active compounds (SAC), a maximum PAHs degradation in BAW (100%) and BAF (86 ± 1.30%) strategies were noted on 21st day. Irrespective of the strategies, presence of SAC and rhamnolipids enhanced PAHs degradation by increasing the enzymes production in Trametes hirsuta when compared to Triton x-100 and sodium dodecyl sulphate (SDS). Among the detected PAHs, 100% degradation within 17 days was noted for naphthalene and acenaphthene in SAC-supplemented BAW system. Further, ecotoxicity analysis established showed the LC50 of sediment soil at 26.5 ± 0.24%, which was reduced by an average of 71% after soil remediation. Hence, the current microcosm system proved that the application of SAC with BAW enhanced the PAHs remediation rate, which supports its application in real time soil remediation.
修复持久性多环芳烃（PAHs）污染土壤已成为近年来的主要挑战。此外，PAHs 修复中常规应用的生物增强策略需要持续供应微生物特定营养素，这使得这些过程不太可行。因此，本研究侧重于使用表面活性剂与木材辅助真菌系统在微宇宙设置中修复 PAHs。在本研究中，在没有表面活性剂的情况下，在添加木材辅助真菌系统（BAW）的生物增强中，PAHs 降解达到饱和，降解率为 61±1.25%，其次是游离真菌系统（BAF）（54±0.46%）。然而，添加 1500mg/L 的表面活性化合物（SAC）后，BAW（100%）和 BAF（86±1.30%）策略在第 21 天达到最大 PAHs 降解。无论采用哪种策略，与 Triton x-100 和十二烷基硫酸钠（SDS）相比，SAC 和鼠李糖脂的存在都增加了糙皮侧耳中的酶产量，从而增强了 PAHs 的降解。在所检测到的 PAHs 中，在添加 SAC 的 BAW 系统中，萘和苊在 17 天内完全降解。此外，生态毒性分析表明，沉积物土壤的 LC50 为 26.5±0.24%，土壤修复后平均降低了 71%。因此，当前的微宇宙系统证明，SAC 与 BAW 的联合应用提高了 PAHs 的修复速率，支持其在实时土壤修复中的应用。
