S2B，一种感染新月柄杆菌 CB15 菌株的温和噬菌体。
S2B, a Temperate Bacteriophage That Infects Caulobacter Crescentus Strain CB15.
机构信息
Department of Biological Sciences, University of South Carolina, Columbia, SC, 29208, US.
Department of Biology, Stanford University, Stanford, CA, 94305, US.
出版信息
Curr Microbiol. 2022 Feb 12;79(4):98. doi: 10.1007/s00284-022-02799-4.
The Caulobacter crescentus strain CB15 has been the basis of numerous studies designed to characterize the biphasic life cycle of this bacterium. Here we describe a newly isolated podovirus, designated S2B, which is capable of integrating into the CB15 chromosome by recombining with the 3'-end of a particular tRNA gene. In addition, we show that S2B is a representative of a family of closely related prophages that are present in the genomes of characterized strains from several Alphaproteobacteria genera. In contrast, only distantly related bacteriophage genomes are present in the GenBank database. The 42,846 bp S2B genome includes 262 bp terminal repeats, and it contains 62 genes of which 45 code for proteins of unknown function. Proteins with predicted functions include a T7 DNA polymerase, a T3/T7 RNA polymerase, and a T7 helicase/primase suggesting that S2B is part of the Studiervirinae subfamily of the Autographiviridae family.
新月柄杆菌 CB15 菌株一直是许多旨在描述该细菌双相生命周期的研究的基础。在这里，我们描述了一种新分离的 Podovirus，命名为 S2B，它能够通过与特定 tRNA 基因的 3'末端重组而整合到 CB15 染色体中。此外，我们表明 S2B 是存在于几种α变形菌属特征菌株基因组中的密切相关原噬菌体家族的代表。相比之下，在 GenBank 数据库中仅存在远缘关系的噬菌体基因组。42846 bp 的 S2B 基因组包括 262 bp 的末端重复序列，它包含 62 个基因，其中 45 个编码未知功能的蛋白质。具有预测功能的蛋白质包括 T7 DNA 聚合酶、T3/T7 RNA 聚合酶和 T7 解旋酶/引发酶，表明 S2B 是 Autographiviridae 家族的 Studiervirinae 亚科的一部分。
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