Department of Ultrasound, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Ultrasound, Ruijin Hospital Luwan Branch, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Aging (Albany NY). 2022 Feb 12;14(3):1389-1406. doi: 10.18632/aging.203884.
Radiofrequency ablation (RFA) is a frequently used thermal ablation technique for breast tumors. The study aimed to identify the effect of sublethal heat treatment on biological function of breast cancer cells and reveal its potential molecular mechanism. The expression profile of dysregulated proteins in sublethal heat treated breast cancer cells was analyzed by quantitative proteomic analysis. The differentially expressed proteins in the sublethal heat treated breast cancer were identified. The potential biological functions of these proteins were evaluated. The proliferation and invasion ability of breast cancer cells were enhanced after sublethal heat treatment. The expression profile of proteins in sublethal heat treated breast cancer cells was abundant, and most of which were newly discovered. A total of 206 differentially expressed proteins were identified. Among them, 101 proteins were downregulated while 105 proteins were upregulated. GO and KEGG analysis indicated that various systems were involved in the process of sublethal heat treatment including cancer, immune system, et al. Immunohistochemistry staining showed that the expression of Heat shock protein 1B, NOB1 and CRIP1 was highly expressed while the expression of BCLAF1 was lower in sublethal heat treated group. The proliferation and invasion ability of breast cancer cells were enhanced after sublethal heat treatment. Sublethal heat treatment caused gene alterations in cancer and immune system. Heat shock protein 1B, NOB1 and CRIP1 were upregulated while BCLAF1 was downregulated in breast cancer after sublethal heat treatment.
射频消融（RFA）是一种常用于治疗乳腺肿瘤的热消融技术。本研究旨在确定亚致死热疗对乳腺癌细胞生物学功能的影响，并揭示其潜在的分子机制。通过定量蛋白质组学分析，分析了亚致死热处理的乳腺癌细胞中失调蛋白的表达谱。鉴定了亚致死热处理的乳腺癌中差异表达的蛋白质。评估了这些蛋白质的潜在生物学功能。亚致死热处理后，乳腺癌细胞的增殖和侵袭能力增强。亚致死热处理的乳腺癌细胞的蛋白质表达谱丰富，其中大多数是新发现的。共鉴定出 206 个差异表达蛋白。其中，101 个蛋白下调，105 个蛋白上调。GO 和 KEGG 分析表明，各种系统参与了亚致死热处理过程，包括癌症、免疫系统等。免疫组织化学染色显示，亚致死热处理组中热休克蛋白 1B、NOB1 和 CRIP1 的表达高度上调，而 BCLAF1 的表达下调。亚致死热处理后，乳腺癌细胞的增殖和侵袭能力增强。亚致死热处理导致癌症和免疫系统的基因改变。亚致死热处理后，乳腺癌中热休克蛋白 1B、NOB1 和 CRIP1 上调，而 BCLAF1 下调。
