Hartshorne T A, Blumberg H, Young E T
Nature. 1986;320(6059):283-7. doi: 10.1038/320283a0.
Classical yeast genetics coupled with the cloning of regulatory genes by complementation of function is a powerful means of identifying and isolating trans-acting regulatory elements. One such regulatory gene is ADR1 which encodes a protein required for transcriptional activation of the glucose-repressible alcohol dehydrogenase (ADH2) gene. We now report the nucleotide sequence of ADR1; it encodes a polypeptide chain of 1,323 amino acids, of which the amino-terminal 302 amino acids are sufficient to stimulate ADH2 transcription. This active amino-terminal region shows amino-acid sequence homology with the repetitive DNA-binding domain of TFIIIA, an RNA polymerase III transcription factor of Xenopus laevis. Similar domains are found in proteins encoded at the Krüppel and Serendipity loci of Drosophila melanogaster. We discuss the implications of this structural homology and suggest that a similar domain may exist in other yeast regulatory proteins such as those encoded by GAL4 (ref. 13) and PPR1 (ref.14).
经典的酵母遗传学方法，再结合通过功能互补克隆调控基因，是鉴定和分离反式作用调控元件的有力手段。一个这样的调控基因是ADR1，它编码葡萄糖可阻遏的乙醇脱氢酶（ADH2）基因转录激活所需的一种蛋白质。我们现在报告ADR1的核苷酸序列；它编码一条由1323个氨基酸组成的多肽链，其中氨基末端的302个氨基酸足以刺激ADH2转录。这个活性氨基末端区域与非洲爪蟾的RNA聚合酶III转录因子TFIIIA的重复DNA结合结构域显示出氨基酸序列同源性。在黑腹果蝇的Krüppel和Serendipity基因座编码的蛋白质中也发现了类似的结构域。我们讨论了这种结构同源性的意义，并提出在其他酵母调控蛋白中可能存在类似的结构域，如由GAL4（参考文献13）和PPR1（参考文献14）编码的那些蛋白。
