生成编码基因编码钙指示剂 RCaMP1h 和 GCaMP6f 的人诱导多能干细胞系。
Generation of human induced pluripotent stem cell lines encoding for genetically encoded calcium indicators RCaMP1h and GCaMP6f.
机构信息
Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Germany.
Department of Human Genetics, Hannover Medical School, Germany.
出版信息
Stem Cell Res. 2022 Apr;60:102697. doi: 10.1016/j.scr.2022.102697. Epub 2022 Feb 2.
Calcium plays a key role in cardiomyocytes (CMs) for the translation of the electrical impulse of an action potential into contraction forces. A rapid, not-invasive fluorescence imaging technology allows for the monitoring of calcium transients in human induced pluripotent stem cell derived-cardiomyocytes (hiPSC-CMs) to investigate the cardiac electrophysiology in vitro and after cell transplantation in vivo. The genetically encoded calcium indicators (GECIs) GCaMP6f or RCaMP1h were successfully transfected in the previously established hiPSC line MHHi001-A, together with a cardiac specific antibiotic selection cassette facilitating the monitoring of the calcium handling in highly pure populations of hiPSC-CMs.
钙在心肌细胞（CMs）中起着关键作用，可将动作电位的电脉冲转化为收缩力。一种快速、非侵入性的荧光成像技术可用于监测人诱导多能干细胞衍生的心肌细胞（hiPSC-CMs）中的钙瞬变，以体外研究心脏电生理学，并在体内细胞移植后进行研究。成功地将基因编码的钙指示剂（GECIs）GCaMP6f 或 RCaMP1h 转染到先前建立的 hiPSC 系 MHHi001-A 中，同时还带有一个心脏特异性抗生素选择盒，便于在 hiPSC-CMs 的高纯度群体中监测钙处理。
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