Clinical oncology department, faculty of medicine, Ain Shams university, Cairo, Egypt.
Pathology department, Faculty of Medicine, Ain Shams university, Cairo, Egypt.
Gulf J Oncolog. 2021 Sep;1(37):23-31.
Tumor microenvironment plays crucial role in cancer evolution. There is a dynamic and continuous relation between immune cells and cancer cells' resistance. Tumor infiltration CD8-lymphocytes and programmed death ligand-1 have proved important prognostic role in different malignancies. We aimed at evaluating this role in laryngeal cancer.
We prospectively analyzed laryngeal cancer patients' specimens, to identify the CD8-lymphocytes and the PD-L1 expression. A total score formed of the sum of percentage and intensity of PD-L1. A final rate was considered as negative or low when combined percentage and intensity scores 0 to 4, and high when scores 5-7. CD8-lymphocyte infiltration was divided into strong (= 10/100 of epithelial cells or =20/100 stromal cell infiltration) or weak (<10/100 epithelial cells or <20/100 stromal cell infiltration).
Forty patients were included; twelve had stage 1 or 2 and 28 with advanced stages. PD-L1 expressionwas positive in 92.5%. Neither the PD-L1 nor CD8- lymphocytes had overall survival impact, however high PD-L1 correlated with better survival in advanced stage subgroup (p = 0.036), high CD8-lymphocytes infiltration had better survival but did not reach significance. Therewas significant correlation between the CD8-lymphocyte infiltration; whether epithelial or stromal, and tumor PD-L1 expression; p-value of 0.001 and < 0.0001 respectively. Subgroup of patients with low CD8+ infiltration and low PD-L1 had the worst survival.
There is a correlation between CD8- l lymphocytes infiltration and PD-L1 expression inlaryngeal cancer and high PD-L1 expression is associated with better OS in advanced stages. Key words: PD-L1, CD8, laryngeal cancer, tumor microenvironment.
肿瘤微环境在癌症演变中起着至关重要的作用。免疫细胞与癌细胞耐药性之间存在着动态的、连续的关系。肿瘤浸润 CD8-淋巴细胞和程序性死亡配体-1（PD-L1）已被证明在不同的恶性肿瘤中有重要的预后作用。我们旨在评估其在喉癌中的作用。
我们前瞻性分析了喉癌患者的标本，以确定 CD8-淋巴细胞和 PD-L1 的表达。一个由 PD-L1 的百分比和强度之和组成的总评分。当组合的百分比和强度评分 0 到 4 时，认为最终评分是阴性或低，当评分 5-7 时认为是高。CD8-淋巴细胞浸润分为强（=上皮细胞的 10/100 或=基质细胞的 20/100 浸润）或弱（<上皮细胞的 10/100 或<基质细胞的 20/100 浸润）。
共纳入 40 例患者，其中 12 例为 1 期或 2 期，28 例为晚期。PD-L1 表达阳性率为 92.5%。PD-L1 和 CD8-淋巴细胞均与总生存无关，但在晚期亚组中，高 PD-L1 与更好的生存相关（p=0.036），高 CD8-淋巴细胞浸润有更好的生存，但无统计学意义。CD8-淋巴细胞浸润无论是上皮细胞还是基质细胞，与肿瘤 PD-L1 表达均有显著相关性；p 值分别为 0.001 和 <0.0001。低 CD8+浸润和低 PD-L1 患者亚组的生存最差。
喉癌中 CD8-淋巴细胞浸润与 PD-L1 表达之间存在相关性，高 PD-L1 表达与晚期患者的 OS 相关。关键词：PD-L1、CD8、喉癌、肿瘤微环境。
