局部振动疗法促进坐骨神经损伤大鼠神经功能的恢复。

Local vibration therapy promotes the recovery of nerve function in rats with sciatic nerve injury.

作者信息

Yin Lu, An Yun, Chen Xiao, Yan Hui-Xin, Zhang Tao, Lu Xin-Gang, Yan Jun-Tao

机构信息

Department of Rehabilitation Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

Department of Tuina, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

出版信息

J Integr Med. 2022 May;20(3):265-273. doi: 10.1016/j.joim.2022.02.001. Epub 2022 Feb 2.

DOI:10.1016/j.joim.2022.02.001

PMID:35153133

Abstract

OBJECTIVE

It has been reported that local vibration therapy can benefit recovery after peripheral nerve injury, but the optimized parameters and effective mechanism were unclear. In the present study, we investigated the effect of local vibration therapy of different amplitudes on the recovery of nerve function in rats with sciatic nerve injury (SNI).

METHODS

Adult male Sprague-Dawley rats were subjected to SNI and then randomly divided into 5 groups: sham group, SNI group, SNI + A-1 mm group, SNI + A-2 mm group, and SNI + A-4 mm group (A refers to the amplitude; n = 10 per group). Starting on the 7th day after model initiation, local vibration therapy was given for 21 consecutive days with a frequency of 10 Hz and an amplitude of 1, 2 or 4 mm for 5 min. The sciatic function index (SFI) was assessed before surgery and on the 7th, 14th, 21st and 28th days after surgery. Tissues were harvested on the 28th day after surgery for morphological, immunofluorescence and Western blot analysis.

RESULTS

Compared with the SNI group, on the 28th day after surgery, the SFIs of the treatment groups were increased; the difference in the SNI + A-2 mm group was the most obvious (95% confidence interval [CI]: [5.86, 27.09], P < 0.001), and the cross-sectional areas of myocytes in all of the treatment groups were improved. The G-ratios in the SNI + A-1 mm group and SNI + A-2 mm group were reduced significantly (95% CI: [-0.12, -0.02], P = 0.007; 95% CI: [-0.15, -0.06], P < 0.001). In addition, the expressions of S100 and nerve growth factor proteins in the treatment groups were increased; the phosphorylation expressions of ERK1/2 protein in the SNI + A-2 mm group and SNI + A-4 mm group were upregulated (95% CI: [0.03, 0.96], P = 0.038; 95% CI: [0.01, 0.94], P = 0.047, respectively), and the phosphorylation expression of Akt in the SNI + A-1 mm group was upregulated (95% CI: [0.11, 2.07], P = 0.031).

CONCLUSION

Local vibration therapy, especially with medium amplitude, was able to promote the recovery of nerve function in rats with SNI; this result was linked to the proliferation of Schwann cells and the activation of the ERK1/2 and Akt signaling pathways.

摘要

目的

据报道，局部振动疗法有助于周围神经损伤后的恢复，但其优化参数和作用机制尚不清楚。在本研究中，我们探讨了不同振幅的局部振动疗法对坐骨神经损伤（SNI）大鼠神经功能恢复的影响。

方法

成年雄性Sprague-Dawley大鼠接受SNI手术，然后随机分为5组：假手术组、SNI组、SNI + A-1 mm组、SNI + A-2 mm组和SNI + A-4 mm组（A表示振幅；每组n = 10）。从模型建立后的第7天开始，连续21天进行局部振动治疗，频率为10 Hz，振幅为1、2或4 mm，持续5分钟。在手术前以及手术后第7、14、21和28天评估坐骨神经功能指数（SFI）。在手术后第28天采集组织进行形态学、免疫荧光和蛋白质印迹分析。

结果

与SNI组相比，手术后第28天，治疗组的SFI升高；SNI + A-2 mm组差异最为明显（95%置信区间[CI]：[5.86, 27.09]，P < 0.001），所有治疗组的肌细胞横截面积均得到改善。SNI + A-1 mm组和SNI + A-2 mm组的G比值显著降低（95% CI：[-0.12, -0.02]，P = 0.007；95% CI：[-0.15, -0.06]，P < 0.001）。此外，治疗组中S100和神经生长因子蛋白的表达增加；SNI + A-2 mm组和SNI + A-4 mm组中ERK1/2蛋白的磷酸化表达上调（分别为95% CI：[0.03, 0.96]，P = 0.038；95% CI：[0.01, 0.94]，P = 0.047），SNI + A-1 mm组中Akt的磷酸化表达上调（95% CI：[0.11, 2.07]，P = 0.031）。