Chen Ming-Zhong, Zhong Xu-Mei, Lin Hai-Sheng, Qin Xiao-Ming
College of Food Science and Technology, and Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Ocean University, Zhanjiang, China.
Yangjiang Polytechnic, Yangjiang, China.
Front Genet. 2022 Jan 27;12:792991. doi: 10.3389/fgene.2021.792991. eCollection 2021.
An increasing attention is being given to treat fruits with ultraviolet C (UV-C) irradiation to extend shelf-life, senescence, and protection from different diseases during storage. However, the detailed understanding of the pathways and key changes in gene expression and metabolite accumulation related to UV-C treatments are yet to be explored. This study is a first attempt to understand such changes in banana peel irradiated with UV-C. We treated Laur. with 0.02 KJ/m UV-C irradiation for 0, 4, 8, 12, 15, and 18 days and studied the physiological and quality indicators. We found that UV-C treatment reduces weight loss and decay rate, while increased the accumulation of total phenols and flavonoids. Similarly, our results demonstrated that UV-C treatment increases the activity of defense and antioxidant system related enzymes. We observed that UV-C treatment for 8 days is beneficial for peels. The peels of treated with UV-C for 8 days were then subjected to combined transcriptome and metabolome analysis. In total, there were 425 and 38 differentially expressed genes and accumulated metabolites, respectively. We found that UV-C treatment increased the expression of genes in secondary metabolite biosynthesis related pathways. Concomitant changes in the metabolite accumulation were observed. Key pathways that were responsive to UV-C irradiation include flavonoid biosynthesis, phenylpropanoid bios6ynthesis, plant-pathogen interaction, MAPK signaling (plant), and plant hormone signal transduction pathway. We concluded that UV-C treatment imparts beneficial effects on banana peels by triggering defense responses against disease, inducing expression of flavonoid and alkaloid biosynthesis genes, and activating phytohormone and MAPK signaling pathways.
人们越来越关注用紫外线C（UV-C）照射处理水果，以延长其保质期、延缓衰老，并在储存期间预防不同疾病。然而，与UV-C处理相关的基因表达和代谢物积累的途径及关键变化的详细情况仍有待探索。本研究首次尝试了解UV-C照射香蕉皮后的此类变化。我们用0.02 KJ/m的UV-C照射香蕉皮0、4、8、12、15和18天，并研究其生理和品质指标。我们发现，UV-C处理可减少重量损失和腐烂率，同时增加总酚和黄酮类化合物的积累。同样，我们的结果表明，UV-C处理可提高与防御和抗氧化系统相关酶的活性。我们观察到，UV-C照射8天对香蕉皮有益。然后对经UV-C照射8天的香蕉皮进行转录组和代谢组联合分析。总共分别有425个和38个差异表达基因及积累的代谢物。我们发现，UV-C处理增加了次生代谢物生物合成相关途径中基因的表达。同时观察到代谢物积累的相应变化。对UV-C照射有反应的关键途径包括黄酮类生物合成、苯丙烷类生物合成、植物-病原体相互作用、MAPK信号传导（植物）和植物激素信号转导途径。我们得出结论，UV-C处理通过触发对疾病的防御反应、诱导黄酮类和生物碱生物合成基因的表达以及激活植物激素和MAPK信号通路，对香蕉皮产生有益影响。
