Yang Yue, Cao Yanan, Qin Gang, Wang Lu, Li Qian, Dai Sisi, Guo Lizhe, Guo Qulian, Peng Yong Gang, Duan Bin, Wang E
Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, People's Republic of China.
Department of Anesthesiology, University of Florida College of Medicine, Gainesville, FL, USA.
Pulm Circ. 2019 Nov 7;9(4):2045894019879393. doi: 10.1177/2045894019879393. eCollection 2019 Oct-Dec.
We performed RNA-sequencing to investigate the changes and expression profiles in long non-coding RNAs (lncRNAs) and their potential functional roles in the lungs of pulmonary arterial hypertension rats responding to acute inflammation.
To establish a pulmonary arterial hypertension rat model, monocrotaline was injected intraperitoneally and lipopolysaccharide was given to induce acute inflammation. Selected lncRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatics analyses were carried out to predict the potential biological roles of key lncRNAs.
Twenty-eight lncRNAs and seven mRNAs with elevated expression and 202 lncRNAs and 36 mRNAs with decreased expression were found in the lung tissues of lipopolysaccharide-treated pulmonary arterial hypertension rats compared with control group. The qRT-PCR validation results were consistent with the bioinformatics analysis. Gene ontology analyses showed that the mRNAs and lncRNAs were differentially expressed in different pathways regarding biological process, cellular components, and molecular function. The functions of differentially expressed messenger RNAs (DEmRNAs) and DElncRNAs were indicated by Kyoto Encyclopedia of Genes and Genomes enrichment.
The DEmRNAs co-expressed with DElncRNAs were obviously enriched in inflammation. DElncRNAs and DEmRNAs in the lungs of pulmonary arterial hypertension rats changed with acute inflammation may provide new insights into the pathogenesis of pulmonary arterial hypertension.
我们进行了RNA测序，以研究长链非编码RNA（lncRNA）的变化和表达谱及其在急性炎症反应的肺动脉高压大鼠肺中的潜在功能作用。
为建立肺动脉高压大鼠模型，腹腔注射野百合碱并给予脂多糖以诱导急性炎症。通过定量实时聚合酶链反应（qRT-PCR）验证所选的lncRNA。进行生物信息学分析以预测关键lncRNA的潜在生物学作用。
与对照组相比，在脂多糖处理的肺动脉高压大鼠肺组织中发现28个lncRNA和7个mRNA表达上调，202个lncRNA和36个mRNA表达下调。qRT-PCR验证结果与生物信息学分析一致。基因本体分析表明，mRNA和lncRNA在生物过程、细胞成分和分子功能的不同途径中差异表达。基因和基因组京都百科全书富集表明了差异表达信使RNA（DEmRNA）和DElncRNA的功能。
与DElncRNA共表达的DEmRNA明显富集于炎症中。急性炎症时肺动脉高压大鼠肺中的DElncRNA和DEmRNA变化可能为肺动脉高压的发病机制提供新的见解。
