Gonçalves Amanda Bezerra, Bovo Júlia Leme, Gomes Bruna Silva, Pigoso Acácio Antonio, Felonato Maíra, Esquisatto Marcelo Augusto Marretto, Filho Gaspar de Jesus Lopes, do Bomfim Fernando Russo Costa
Postgraduate Program in Biomedical Sciences of Centro Universitário da Fundação Hermínio Ometto, Araras, Brazil.
Postgraduate Program in Interdisciplinary Surgical Science, Universidade Federal de São Paulo, UNIFESP-EPM, São Paulo, Brazil.
J Lasers Med Sci. 2021 Oct 18;12:e60. doi: 10.34172/jlms.2021.60. eCollection 2021.
Rheumatoid arthritis (RA) causes inflammation, pain, edema, and articular degradation and its treatment can be based on anti-inflammatory drugs, photobiomodulation (PBM) and/or platelet-rich plasma (PRP) that can decrease cell flow and promote local healing. In the present study, we evaluate the effects of PBM and PRP on acute arthritis in Wistar rats through inflammatory and oxidative stress parameters. Thirty female Wistar rats were assigned to five groups (n=6, each group): Control, Sham, PRP, Laser, and PRP+Laser. For arthritis induction, all animals of groups Sham, PRP, Laser and PRP+Laser received an intraarticular injection of Zymosan® (200µg) in the right knee. Twenty-four hours post-arthritis induction, PRP was prepared and injected (8 × 10 of platelets) in animals of PRP and PRP+Laser groups. PBM was performed in Laser and PRP+Laser groups by single-dose therapy with the GaAlAs laser (λ=808 nm, P=25 mW, fluence=30 J/cm, beam area=0.02 mm, t=33 seconds, E=0.825 J, punctual application). After seven days of induction, serum samples were collected and thiobarbituric acid reactive substances (TBARS), nitric oxide (NO) and catalase activity were analysed. Morphological parameters were measured for inflammation areas, cartilage thickness, and C3 protein expression in knee samples. Statistical analysis was performed with an ANOVA test and Tukey's post-hoc test with a significance level of 5% (<0.05). NO was lower in the treated groups compared to the Sham group, and TBARS did not show any differences, while catalase showed greater activity between PRP+Laser versus PRP (<0.05). Inflammatory areas and cartilage thickness were lower in the treated groups compared to Sham (<0.05), while no differences in C3 protein expression was observed. PBM associated with PRP is better for anti-inflammatory and joint preservation by morphological aspects and NO levels that concern a potential clinical application.
类风湿性关节炎（RA）会引发炎症、疼痛、水肿和关节退化，其治疗可基于抗炎药物、光生物调节（PBM）和/或富含血小板血浆（PRP），这些方法可减少细胞流动并促进局部愈合。在本研究中，我们通过炎症和氧化应激参数评估PBM和PRP对Wistar大鼠急性关节炎的影响。将30只雌性Wistar大鼠分为五组（每组n = 6）：对照组、假手术组、PRP组、激光组和PRP + 激光组。为诱导关节炎，假手术组、PRP组、激光组和PRP + 激光组的所有动物右膝均接受关节内注射酵母聚糖（200μg）。关节炎诱导后24小时，制备PRP并注射到PRP组和PRP + 激光组动物体内（血小板浓度为8×10）。激光组和PRP + 激光组采用GaAlAs激光进行单剂量PBM治疗（λ = 808 nm，P = 25 mW，能量密度 = 30 J/cm，光斑面积 = 0.02 mm，t = 33秒，E = 0.825 J，点状照射）。诱导7天后，收集血清样本并分析硫代巴比妥酸反应性物质（TBARS）、一氧化氮（NO）和过氧化氢酶活性。测量膝关节样本中炎症区域、软骨厚度和C3蛋白表达的形态学参数。采用方差分析（ANOVA）检验和Tukey事后检验进行统计分析，显著性水平为5%（<0.05）。与假手术组相比，治疗组的NO水平较低，TBARS无差异，而过氧化氢酶在PRP + 激光组与PRP组之间活性更高（<0.05）。与假手术组相比，治疗组的炎症区域和软骨厚度更低（<0.05），而C3蛋白表达无差异。从形态学方面和与潜在临床应用相关的NO水平来看，PRP与PBM联合使用在抗炎和关节保护方面效果更佳。
