Department of Physiology, College of Medicine, Wasit University, Kut, Iraq.
Department of Basic Science, College of Dentistry, Ibn Sina University of Medical and Pharmaceutical Sciences, Baghdad, Iraq.
Lasers Med Sci. 2021 Mar;36(2):357-363. doi: 10.1007/s10103-020-03039-6. Epub 2020 May 21.
There were many studies that attempt to measure the effect of growth factors of platelets through platelet-rich plasma (PRP) techniques on repairing of different human tissues and their efficiency either by platelets account or measuring the concentrations of growth factors secreted from platelets at various experimental conditions, to get the optimal parameters for platelets functions in healing processes. There were little trails dealing with laser and PRP for accelerating healing process that generally takes two methods, either by studding the stimulation effect of LLLT (low-level laser therapy), by subjecting laser irradiation on injured part and left for a period of time that is necessary for photobiostimulation of cell proliferations, then PRP treatment followed, or by studding the direct effects of laser on PRP factors activity. The objectives of this study are to investigate the indirect and prolonged influence of laser irradiation (650 nm with 100 mW output power) on healing processes of knee joints with induced osteoarthritis (OA), by comparison of radiated and non-radiated PRP on repairing of joint cartilage. In material and methods, we used 9 rats divided in to four groups: C1, control without any treatment, for positive comparisons of healing; C2 and C3, controls with induced OA, left for 14 days, then sacrificed for histological analysis of negative comparisons; and P and L groups that had induced with OA for 14 days and then treated with non-irradiated and radiated PRP, respectively. Preparation of PRP (condensed platelets account with high concentration of growth factors) in order to accelerate repairing processes on induced- osteoarthritis cartilage in rats groups. To estimate the efficacy of photobiostimulation or photobioinhibition on platelets' granules, we determine the absorbance of PRP by spectrophotometer. The technique was based on PRP, as a feature of platelets quantity, that compares the quality of PRP on healing of induced osteoarthritis with and without irradiation of laser, using Wistar rats as a model. The quality of platelets was measured by time required for healing according to histopathological observations and grades of OA. Finally, the results were analyzed statistically using ANOVA test (P = 0.05). Our conclusion was emphasizing the idea of inhibiting the effect of LLLT on growth factors of PRP that is responsible of speed up healing of OA.
有许多研究试图通过富含血小板的血浆 (PRP) 技术来测量血小板生长因子对不同人体组织的修复效果，并通过血小板计数或测量在各种实验条件下从血小板分泌的生长因子的浓度来衡量其效率，以获得血小板在愈合过程中发挥作用的最佳参数。有一些关于激光和 PRP 加速愈合过程的试验，这些试验通常有两种方法，一种是研究低水平激光疗法 (LLLT) 的刺激作用，通过将激光照射到受伤部位并保持一段时间，以促进细胞增殖的光生物刺激，然后进行 PRP 治疗，或者研究激光对 PRP 因子活性的直接影响。本研究的目的是通过比较放射和未放射的 PRP 对关节软骨修复的影响，研究激光照射对诱导性骨关节炎 (OA) 膝关节愈合过程的间接和长期影响。在材料和方法中，我们使用了 9 只大鼠，分为 4 组：C1 组，未接受任何治疗，作为愈合的阳性对照；C2 组和 C3 组，诱导 OA，放置 14 天，然后进行组织学分析作为阴性对照；P 组和 L 组，诱导 OA 14 天后，分别接受非照射和照射的 PRP 治疗。PRP（血小板浓缩物，具有高浓度的生长因子）的制备，以加速大鼠诱导性 OA 软骨的修复过程。为了估计光生物刺激或光生物抑制对血小板颗粒的影响，我们通过分光光度计测定 PRP 的吸光度。该技术基于 PRP，作为血小板数量的特征，比较了有无激光照射的 PRP 对诱导性 OA 愈合的质量，使用 Wistar 大鼠作为模型。血小板的质量通过根据组织病理学观察和 OA 分级所需的愈合时间来测量。最后，使用方差分析 (ANOVA) 检验对结果进行统计学分析 (P = 0.05)。我们的结论强调了抑制 LLLT 对 PRP 生长因子的影响的想法，因为生长因子有助于加速 OA 的愈合。
