Tylosin Inhibits Biofilm Formation by Interacting With the O-acetylserine (thiol)-lyase B CysM.

() can decrease its virulence or modify local conditions through biofilm formation, which promotes infection persistence . Biofilm formation is an important cause of chronic drug-resistant infection. The aim of this study was to evaluate whether tylosin effectively inhibits biofilm formation by interacting with O-acetylserine (thiol)-lyase B (CysM), a key enzymatic regulator of cysteine synthesis. Biofilm formation of the mutant (ΔcysM) strain was significantly lower compared to the wild-type ATCC 700794 strain. Tylosin inhibited gene expression, decreased extracellular matrix contents, and reduced cysteine, homocysteine, and S-adenosylmethionine levels, indicating its potential value as an effective inhibitor of biofilm formation. Furthermore, using biolayer interferometry technology and fourier-transform infrared spectroscopy, we found that tylosin and CysM could be combined directly. Overall, these results provide evidence that tylosin inhibits biofilm formation by interacting with CysM.