CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 101408, China.
Int J Mol Sci. 2022 Aug 20;23(16):9399. doi: 10.3390/ijms23169399.
is a widely distributed marine bacterium that is a threat to the aquaculture industry as well as human health. Evidence has revealed critical roles for small RNAs (sRNAs) in bacterial physiology and cellular processes by modulating gene expression post-transcriptionally. GcvB is one of the most conserved sRNAs that is regarded as the master regulator of amino acid uptake and metabolism in a wide range of Gram-negative bacteria. However, little information about GcvB-mediated regulation in is available. Here we first characterized GcvB in ZJ-T and determined its regulon by integrated transcriptome and quantitative proteome analysis. Transcriptome analysis revealed 40 genes differentially expressed (DEGs) between wild-type ZJ-T and mutant ZJ-T-Δ, while proteome analysis identified 50 differentially expressed proteins (DEPs) between them, but only 4 of them displayed transcriptional differences, indicating that most DEPs are the result of post-transcriptional regulation of . Among the differently expressed proteins, 21 are supposed to be involved in amino acid biosynthesis and transport, and 11 are associated with type three secretion system (T3SS), suggesting that GcvB may play a role in the virulence besides amino acid metabolism. RNA-EMSA showed that Hfq binds to GcvB, which promotes its stability.
是一种广泛分布的海洋细菌，对水产养殖业以及人类健康构成威胁。有证据表明，小 RNA（sRNA）通过转录后调控基因表达，在细菌生理学和细胞过程中发挥着重要作用。GcvB 是最保守的 sRNA 之一，被认为是革兰氏阴性菌中氨基酸摄取和代谢的主要调节剂。然而，关于 GcvB 在 中的介导调控的信息很少。在这里，我们首次在 ZJ-T 中对 GcvB 进行了表征，并通过整合转录组和定量蛋白质组分析确定了其调控组。转录组分析显示，野生型 ZJ-T 和 突变型 ZJ-T-Δ 之间有 40 个基因差异表达（DEGs），而蛋白质组分析则在它们之间鉴定出 50 个差异表达蛋白（DEPs），但只有其中 4 个显示转录差异，表明大多数 DEPs 是 的转录后调控的结果。在差异表达的蛋白质中，有 21 个被认为参与氨基酸的生物合成和运输，有 11 个与 III 型分泌系统（T3SS）有关，这表明 GcvB 除了在氨基酸代谢中可能发挥作用外，还可能在毒力中发挥作用。RNA-EMSA 表明 Hfq 与 GcvB 结合，从而促进其稳定性。
