Noble Research Institute, LLC, Ardmore, OK 73401, U.S.A.
Department of Plant Pathology, Washington State University, Mount Vernon NWREC, Mount Vernon, WA 98273, U.S.A.
Plant Dis. 2022 Aug;106(8):2105-2116. doi: 10.1094/PDIS-01-22-0013-RE. Epub 2022 Jul 18.
is a member of Pezizomycetes and causes root rot disease on a broad range of dicotyledonous plants. Using recently generated draft genome sequence data from four isolates, we developed simple sequence repeat (SSR) markers and identified both mating type genes ( and ) in this fungus. To understand the genetic diversity of isolates ( = 43) and spore mats ( = 29) collected from four locations (Oklahoma, Texas, Arizona, and Mexico) and four host crops (cotton, alfalfa, peach, and soybean), we applied 24 SSR markers and showed that of the 72 isolates and spore mats tested, 41 were distinct genotypes. Furthermore, the developed SSR markers did not show cross-transferability to other close relatives of in the class Pezizomycetes. A multiplex PCR detecting both mating type idiomorphs and a reference gene () was developed to screen isolates. Based on the dataset we tested, is a heterothallic fungus with both mating types present in the United States in a ratio close to 1:1. We tested spore mats obtained from spatially distinct disease rings that developed in a center-pivot alfalfa field and showed that both mating types can be present not only in the same field but also within a single spore mat. This study shows that has the genetic toolkit for generating sexually diverse progeny, providing impetus for future studies that focus on identifying sexual morphs in nature.
是子囊菌门的一员，可引起广泛的双子叶植物根腐病。利用最近从四个分离株生成的草图基因组序列数据，我们开发了简单重复序列（SSR）标记，并在该真菌中鉴定了两个交配型基因（和）。为了了解来自四个地点（俄克拉荷马州、德克萨斯州、亚利桑那州和墨西哥）和四个宿主作物（棉花、紫花苜蓿、桃树和大豆）的 分离株（=43）和孢子垫（=29）的遗传多样性，我们应用了 24 个 SSR 标记，结果表明在 72 个测试的分离株和孢子垫中，有 41 个是独特的基因型。此外，开发的 SSR 标记不能转移到子囊菌门中与 关系密切的其他近缘种。我们开发了一种检测两个交配型单倍型和一个参考基因（）的多重 PCR 来筛选 分离株。根据我们测试的数据集， 是一种异宗配合真菌，在美国两种交配型的比例接近 1:1。我们测试了从中心枢轴紫花苜蓿田中发育的空间上不同的病害环中获得的 孢子垫，结果表明两种交配型不仅可以存在于同一田间，而且可以存在于单个孢子垫中。这项研究表明， 具有产生性差异后代的遗传工具包，为未来在自然界中识别有性形态的研究提供了动力。
