Mackie D P, Meneely D J, Pollock D A, Logan E F
Vet Immunol Immunopathol. 1986 Feb;11(2):193-8. doi: 10.1016/0165-2427(86)90097-8.
The role of the IgA antibody to Streptococcus agalactiae found in the whey of milks 12 hours after the first intramammary infection of six Friesian first lactation heifers was assessed using an in vitro bactericidal assay. The mean percentage kill of the streptococci by neutrophils in the presence of these wheys was 36.2% while the equivalent figure for the non-infected quarter whey was 0%. When the IgA antibody was absorbed from the infected quarter wheys using class specific IgA antiserum cross linked with glutaraldehyde the percentage kill of the test system fell to 0%. Elution of the absorbed antibody partially restored the activity to a mean percentage kill of 18.2%. The results indicated that the IgA antibody found in infected quarter whey during the acute stages of intramammary infection with Streptococcus agalactiae was responsible for the opsonic activity which pertained at that time.
采用体外杀菌试验，评估了在6头弗里生初产奶牛首次乳房内感染12小时后乳汁乳清中发现的抗无乳链球菌IgA抗体的作用。在这些乳清存在的情况下，中性粒细胞对链球菌的平均杀灭率为36.2%，而未感染乳腺的乳清的相应杀灭率为0%。当使用与戊二醛交联的类特异性IgA抗血清从感染乳腺的乳清中吸收IgA抗体时，测试系统的杀灭率降至0%。洗脱吸收的抗体可使活性部分恢复，平均杀灭率达到18.2%。结果表明，在无乳链球菌乳房内感染急性期，感染乳腺的乳清中发现的IgA抗体具有当时存在的调理活性。
