Niazi Tabar Amirreza, Azizi Hossein, Hashemi Karoii Danial, Skutella Thomas
Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol 4616849767, Iran.
Institute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany.
Animals (Basel). 2022 Jan 22;12(3):268. doi: 10.3390/ani12030268.
Vimentin is a type of intermediate filament (IF) and one of the first filaments expressed in spermatogenesis. Vimentin plays numerous roles, consisting of the determination of cell shape, differentiation, cell motility, the maintenance of cell junctions, intracellular trafficking, and assisting in keeping normal differentiating germ cell morphology. This study investigated the vimentin expression in two populations of undifferentiated and differentiated spermatogonia. We examined vimentin expression in vivo and in vitro by immunocytochemistry (ICC), immunohistochemistry (IMH), and Fluidigm real-time polymerase chain reaction. IMH data showed that the high vimentin expression was localized in the middle of seminiferous tubules, and low expression was in the basal membrane. ICC analysis of the colonies by isolated differentiated spermatogonia indicated the positive expression for the vimentin antibody, but vimentin's expression level in the undifferentiated population was negative under in vitro conditions. Fluidigm real-time PCR analysis showed significant vimentin expression in differentiated spermatogonia compared to undifferentiated spermatogonia ( < 0.05). Our results showed that vimentin is upregulated in the differentiation stages of spermatogenesis, proving that vimentin is an intermediate filament with crucial roles in the differentiation stages of testicular germ cells. These results support the advanced investigations of the spermatogenic process, both in vitro and in vivo.
波形蛋白是一种中间丝（IF），是精子发生过程中最早表达的丝状物之一。波形蛋白发挥着多种作用，包括确定细胞形状、分化、细胞运动、维持细胞连接、细胞内运输以及协助保持正常分化的生殖细胞形态。本研究调查了波形蛋白在未分化和分化精原细胞两个群体中的表达情况。我们通过免疫细胞化学（ICC）、免疫组织化学（IMH）和Fluidigm实时聚合酶链反应在体内和体外检测波形蛋白的表达。IMH数据显示，波形蛋白高表达定位于生精小管中部，低表达位于基底膜。对分离的分化精原细胞集落进行ICC分析表明波形蛋白抗体呈阳性表达，但在体外条件下，未分化群体中波形蛋白的表达水平为阴性。Fluidigm实时PCR分析显示，与未分化精原细胞相比，分化精原细胞中波形蛋白表达显著（<0.05）。我们的结果表明，波形蛋白在精子发生的分化阶段上调，证明波形蛋白是一种在睾丸生殖细胞分化阶段起关键作用的中间丝。这些结果支持了对精子发生过程在体内和体外的进一步研究。
