Zhou Chang-Qing, Ka Wei, Zhang Hui-Jun, Li Ya-Lan, Gao Pan, Long Rui-Jun, Yang Shun-Wen, Wang Jian-Lin
State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Grassland Agriculture Engineering Center, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.
School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Animals (Basel). 2022 Jan 29;12(3):325. doi: 10.3390/ani12030325.
As the global climate warms, more creatures are threatened by high temperatures, especially cold-water fish such as rainbow trout. Evidence has demonstrated that long noncoding RNAs (lncRNAs) play a pivotal role in regulating heat stress in animals, but we have little understanding of this regulatory mechanism. The present study aimed to identify potential key lncRNAs involved in regulating acute heat stress in rainbow trout. lncRNA and mRNA expression profiles of rainbow trout head kidney were analyzed via high-throughput RNA sequencing, which exhibited that 1256 lncRNAs (802 up-regulation, 454 down-regulation) and 604 mRNAs (353 up-regulation, 251 down-regulation) were differentially expressed. These differentially expressed genes were confirmed to be primarily associated with immune regulation, apoptosis, and metabolic process signaling pathways through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and coding-noncoding co-expression network analysis. These results suggested that 18 key lncRNA-mRNA pairs are essential in regulating acute heat stress in rainbow trout. Overall, these analyses showed the effects of heat stress on various physiological functions in rainbow trout at the transcriptome level, providing a theoretical basis for improving the production and breeding of rainbow trout and the selection of new heat-resistant varieties.
随着全球气候变暖，越来越多的生物受到高温威胁，尤其是虹鳟等冷水鱼。有证据表明，长链非编码RNA（lncRNA）在调节动物热应激中起关键作用，但我们对这种调节机制了解甚少。本研究旨在鉴定参与调节虹鳟急性热应激的潜在关键lncRNA。通过高通量RNA测序分析虹鳟头肾的lncRNA和mRNA表达谱，结果显示有1256个lncRNA（802个上调，454个下调）和604个mRNA（353个上调，251个下调）差异表达。通过基因本体论和京都基因与基因组百科全书通路富集分析以及编码-非编码共表达网络分析，证实这些差异表达基因主要与免疫调节、细胞凋亡和代谢过程信号通路相关。这些结果表明，18对关键lncRNA-mRNA对在调节虹鳟急性热应激中至关重要。总体而言，这些分析在转录组水平上显示了热应激对虹鳟各种生理功能的影响，为提高虹鳟的生产和育种以及新耐热品种的选育提供了理论依据。
