嗜酸氧化亚铁硫杆菌中的跨膜电势和跨膜pH梯度
Transmembrane electrical potential and transmembrane pH gradient in the acidophile Thiobacillus ferro-oxidans.
作者信息
Cox J C, Nicholls D G, Ingledew W J
出版信息
Biochem J. 1979 Jan 15;178(1):195-200. doi: 10.1042/bj1780195.
Abstract
Thiobacillus ferro-oxidans is capable of using the oxidation of Fe2+ by O2 at pH 2.0 as the sole source of energy for growth and CO2 fixation. The bacterium maintains an intracellular pH of 6.5 over a range of external pH from 1.0 to 8.0, as measured by [14C]acetate and [3H]methylamine distribution. The membrane potential was estimated by the distribution of the lipid-soluble cation dibenzyldimethylammonium and the anion SCN-. At pH 2.0 (the pH of growth) during Fe2+ oxidation the transmembrane pH gradient is 4.5 units with an opposing membrane potential of -10mV, giving a proton electrochemical gradient of +256mV. This gradient is actively maintained.
摘要
氧化亚铁硫杆菌能够利用在pH 2.0条件下氧气将Fe2+氧化的过程作为生长和固定二氧化碳的唯一能量来源。通过[14C]乙酸盐和[3H]甲胺分布测定，该细菌在1.0至8.0的外部pH范围内维持细胞内pH为6.5。通过脂溶性阳离子二苄基二甲基铵和阴离子SCN-的分布来估计膜电位。在Fe2+氧化过程中pH 2.0（生长pH值）时，跨膜pH梯度为4.5个单位，相反的膜电位为-10mV，质子电化学梯度为+256mV。这个梯度是被主动维持的。
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