Honda K, Miyaguchi K, Nishino H, Tanaka H, Yao T, Imai K
Anal Biochem. 1986 Feb 15;153(1):50-3. doi: 10.1016/0003-2697(86)90059-x.
A sensitive and selective method for the simultaneous determination of acetylcholine (ACh) and choline (Ch) is reported. ACh and Ch were separated on a reversed-phase column, passed through an immobilized enzymes (acetylcholine esterase and choline oxidase) column, and converted to hydrogen peroxide. The generated hydrogen peroxide was detected by the peroxyoxalate chemiluminescence reaction. The linear determination ranges were from 10 pmol to 10 nmol. The detection limit for both cholines was 1 pmol.
报道了一种同时测定乙酰胆碱（ACh）和胆碱（Ch）的灵敏且选择性的方法。ACh和Ch在反相柱上分离，通过固定化酶（乙酰胆碱酯酶和胆碱氧化酶）柱，并转化为过氧化氢。产生的过氧化氢通过过氧草酸酯化学发光反应进行检测。线性测定范围为10皮摩尔至10纳摩尔。两种胆碱的检测限均为1皮摩尔。
