保加利亚代表性物种的DNA条形码研究。

DNA Barcoding Study of Representative Species in Bulgaria.

作者信息

Aneva Ina, Zhelev Petar, Bonchev Georgi, Boycheva Irina, Simeonova Stiliana, Kancheva Denitsa

机构信息

Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.

Department of Dendrology, University of Forestry, 1797 Sofia, Bulgaria.

出版信息

Plants (Basel). 2022 Jan 20;11(3):270. doi: 10.3390/plants11030270.

DOI:10.3390/plants11030270

PMID:35161250

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8840554/

Abstract

We present a study on the taxonomy of eleven species, belonging to two sections and occurring naturally in Bulgaria. Four DNA barcoding markers-matK, rbcL, trnH-psbA and ITS-were applied to discriminate the species and to reveal their phylogenetic relationships. The results showed that rbcL has the lowest discriminating power regarding the studied species, while the other markers yielded results fitting better to the existing taxonomic schemes based on morphological traits. However, even in the case of better performing markers, the results were not straightforward-morphologically distinct species belonging to different sections were grouped together, and closely related species appeared genetically distinct. The results are typical for taxonomically complex groups, such as the genus , characterized in Bulgaria with great diversity, high percentage of endemism and still requiring a full and comprehensive taxonomic study. The results are discussed in the light of unresolved taxonomic problems and application of DNA barcoding methods.

摘要

我们展示了一项关于11个物种分类学的研究，这些物种属于两个组，自然生长在保加利亚。应用了四个DNA条形码标记——matK、rbcL、trnH-psbA和ITS，以区分这些物种并揭示它们的系统发育关系。结果表明，rbcL对所研究物种的鉴别能力最低，而其他标记产生的结果与基于形态特征的现有分类方案更相符。然而，即使在表现较好的标记的情况下，结果也并非一目了然——属于不同组的形态上不同的物种被归在一起，而亲缘关系密切的物种在基因上却表现出差异。这些结果对于分类学复杂的类群来说是典型的，比如该属，在保加利亚具有高度多样性、高比例的特有性，并且仍然需要进行全面和综合的分类学研究。根据未解决的分类学问题和DNA条形码方法的应用对结果进行了讨论。