Golosow L, Hulme R, Cho S, Vermeulen C W
Biochem Biophys Res Commun. 1986 Mar 28;135(3):708-12. doi: 10.1016/0006-291x(86)90986-1.
Steady state cultures of Escherichia coli W3110 possess constant cellular concentrations of ribosomes while above 22 C as measured isopycnographically. Below 22 C, ribosome levels are significantly diminished, equivalent to those found in dormant cells. Concommitantly, the energy of activation of steady state population growth in E. coli remains at a constant 18.6 kcal between 22 C and 37 C. This suggests that the thermal stability of the protein synthesis apparatus may play an important role in pathogenesis as well as in other areas of ecological niche acquisition and dominance.
通过等密度离心法测量发现，在22℃以上时，大肠杆菌W3110的稳态培养物中核糖体的细胞浓度保持恒定。在22℃以下，核糖体水平显著降低，与休眠细胞中的水平相当。与此同时，大肠杆菌稳态群体生长的活化能在22℃至37℃之间保持在18.6千卡的恒定水平。这表明蛋白质合成装置的热稳定性可能在发病机制以及生态位获取和主导的其他领域中发挥重要作用。
