Department of General, Gastroenterological, and Oncological Surgery Collegium Medicum, Nicolaus Copernicus University, Gagarina 7, 87-100 Toruń, Poland.
Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Toruń, Wileńska 4 Str., 87-100 Toruń, Poland.
Int J Environ Res Public Health. 2022 Feb 6;19(3):1833. doi: 10.3390/ijerph19031833.
The aim of this study was to develop an innovative method of examining bacterial survival using capillary zone electrophoresis (CZE) and flow cytometry (FC) as a reference method. For this purpose, standard strains of bacteria from the ATCC collection were used: ATCC 14506, ATCC 11632, ATCC 10031, ATCC 27853, and ATCC 25922, as well as seven antibiotics with different antimicrobial mechanisms of action. The ratio of live and dead cells in the tested sample in CZE measurements were calculated using our algorithm that takes into account the detection time. Results showed a high agreement between CZE and FC in the assessment of the percentage of live cells exposed to the stress factor in both antibiotic susceptibility and time-dependent assays. The applied measuring system to assess the effectiveness of antibiotic therapy in in vitro conditions is a method with great potential, and the data obtained with the use of CZE mostly correspond to the expected drug sensitivity according to EUCAST and CLSI guidelines.
本研究旨在开发一种创新的细菌存活检测方法，使用毛细管区带电泳（CZE）和流式细胞术（FC）作为参考方法。为此，使用了 ATCC 收藏的标准菌株：ATCC 14506、ATCC 11632、ATCC 10031、ATCC 27853 和 ATCC 25922，以及七种具有不同抗菌作用机制的抗生素。在 CZE 测量中，使用我们的算法计算测试样品中活细胞和死细胞的比例，该算法考虑了检测时间。结果表明，在评估抗生素药敏试验和时间依赖性试验中暴露于应激因素的活细胞百分比方面，CZE 和 FC 之间具有高度一致性。在体外条件下评估抗生素治疗效果的应用测量系统是一种具有巨大潜力的方法，并且使用 CZE 获得的数据与 EUCAST 和 CLSI 指南根据预期药物敏感性大多相符。
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