State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
Food Chem. 2020 Aug 1;320:126581. doi: 10.1016/j.foodchem.2020.126581. Epub 2020 Mar 10.
Foodborne bacterial infection poses a serious threat to human health. As most diseases are caused by living bacteria, real-time assessment of bacterial viability is vitally important to the public health sector. Herein, we developed a simple and novel colorimetric assay based on the Glucose oxidase (GOD)/Horseradish peroxidase (HRP) bienzyme system for real-time monitoring of bacterial viability in food and drinking water. This bienzyme system is free of any chemical synthesis and only requires 3 sample handling steps. The color response is easily observable with the naked eye or recordable with a smartphone for precise determination of bacterial viability. The proposed strategy was validated with various bacteria both Gram-positive and Gram-negative, indicating its capability for broad-spectrum bacteria viability detection. Therefore, the proposed strategy shows promise for rapid and reliable quality control in food and drinking water.
食源性细菌感染对人类健康构成严重威胁。由于大多数疾病是由活细菌引起的，因此实时评估细菌的生存能力对公共卫生部门至关重要。在此，我们开发了一种基于葡萄糖氧化酶（GOD）/辣根过氧化物酶（HRP）双酶系统的简单新颖比色测定法，用于实时监测食品和饮用水中的细菌活力。该双酶系统无需任何化学合成，仅需 3 个样品处理步骤。颜色反应用肉眼即可轻松观察到，也可以用智能手机记录下来，从而可以精确地确定细菌的生存能力。该方法已用各种革兰氏阳性和革兰氏阴性细菌进行了验证，表明其具有广谱细菌生存能力检测的能力。因此，该方法有望在食品和饮用水的快速可靠质量控制方面发挥作用。
