Centre National de Recherche en Génomique Humaine, CEA, Université Paris-Saclay, 91057 Evry, France.
Laboratoire Européen de Recherche pour la Polyarthrite Rhumatoïde-Genhotel, Université Paris-Saclay, Univ Evry, 91042 Evry, France.
Int J Mol Sci. 2022 Jan 20;23(3):1115. doi: 10.3390/ijms23031115.
Understanding the emergence of lymphoid committed cells from multipotent progenitors (MPP) is a great challenge in hematopoiesis. To gain deeper insight into the dynamic expression changes associated with these transitions, we report the quantitative transcriptome of two MPP subsets and the common lymphoid progenitor (CLP). While the transcriptome is rather stable between MPP2 and MPP3, expression changes increase with differentiation. Among those, we found that pioneer lymphoid genes such as , , and are expressed continuously from MPP2. Others, such as CD93, are CLP specific, suggesting their potential use as new markers to improve purification of lymphoid populations. Notably, a six-transcription factor network orchestrates the lymphoid differentiation program. Additionally, we pinpointed 24 long intergenic-non-coding RNA (lincRNA) differentially expressed through commitment and further identified seven novel forms. Collectively, our approach provides a comprehensive landscape of coding and non-coding transcriptomes expressed during lymphoid commitment.
理解淋巴样定向细胞从多能祖细胞（MPP）的出现是造血中的一个巨大挑战。为了更深入地了解与这些转变相关的动态表达变化，我们报告了两个 MPP 亚群和共同淋巴样祖细胞（CLP）的定量转录组。虽然 MPP2 和 MPP3 之间的转录组相当稳定，但分化过程中的表达变化会增加。在这些变化中，我们发现祖细胞样淋巴样基因，如 、 和 ，从 MPP2 开始连续表达。其他基因，如 CD93，是 CLP 特异性的，这表明它们可能被用作新的标记物来提高淋巴样群体的纯化度。值得注意的是，一个六转录因子网络协调了淋巴样分化程序。此外，我们还确定了 24 个在定向过程中差异表达的长非编码 RNA（lncRNA），并进一步鉴定了 7 种新的形式。总的来说，我们的方法提供了淋巴定向过程中表达的编码和非编码转录组的全面图谱。
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